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Dectin-1内在化表达介导氧依赖性方式杀伤白色念珠菌
董碧麟1; 李东升1; 段逸群1; 柳卫凰2; 陈金波1; 刘伟3
2012
Source Publication中华微生物学和免疫学杂志
ISSN0254-5101
Volume32.0Issue:007Pages:577
Abstract目的探讨Dectin-1介导人嗜中性粒细胞以氧依赖性方式杀伤调理吞噬后白色念珠菌孢子的机制。方法在白色念珠菌孢子作用前后,流式细胞术和激光共聚焦显微镜检测人嗜中性粒细胞中Dectin.1的表达率及表达部位;并通过Dectin-1阻断试验分析嗜中性粒细胞中Dectin-1的表达与胞内活性氧簇(reactiveoxygenspecies,ROS)的产生及对白色念珠菌孢子杀伤的相关性。结果在白色念珠菌孢子刺激30或60min后,细胞内Dectin-1的表达率明显上调(0min,8.32%;30min,16.82%;60rain,23.88%。与0min相比,P均〈0.01),且Dectin-1与ROS在胞内的表达均被募集到吞噬的白色念珠菌孢子表面。Dectin-1的阻断分别与ROS峰值的抑制(R2=0.306,P〈0.01)及白色念珠菌杀伤率的降低(R2=0.251,P〈0.01)呈剂量依存关系。结论Dectin-1可通过内在化的表达模式介导人嗜中性粒细胞以ROS依赖性方式杀伤调理吞噬的白色念珠菌孢子。
Keyword人嗜中性粒细胞 白色念珠菌 活性氧簇 Dectin-1
Language英语
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/42462
Collection其他_期刊论文
Affiliation1.湖北武汉市中西医结合医院
2.武汉大学
3.中国科学院水生生物研究所
Recommended Citation
GB/T 7714
董碧麟,李东升,段逸群,等. Dectin-1内在化表达介导氧依赖性方式杀伤白色念珠菌[J]. 中华微生物学和免疫学杂志,2012,32.0(007):577.
APA 董碧麟,李东升,段逸群,柳卫凰,陈金波,&刘伟.(2012).Dectin-1内在化表达介导氧依赖性方式杀伤白色念珠菌.中华微生物学和免疫学杂志,32.0(007),577.
MLA 董碧麟,et al."Dectin-1内在化表达介导氧依赖性方式杀伤白色念珠菌".中华微生物学和免疫学杂志 32.0.007(2012):577.
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