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棉铃虫病毒HaSNPV fp25k基因的克隆表达及抗体制备
吴东; 邓菲; 胡志红; 袁丽; 孙修炼
2004
Source Publication中国病毒学
ISSN1003-5125
Volume19.0Issue:004Pages:380
Abstract根据棉铃虫单核衣壳核多角体病毒(Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus,HaSNPV)fp25k基因的序列。设计引物,引入适当的酶切位点,利用PCR扩增出基因片段。将该基因片段克隆至原核表达载体pProEXHTb,经IFTG诱导,在大肠杆菌DH5α获得了高效表达,表达产物的大小为32kDa。纯化蛋白产物免疫家兔制备抗血清。该抗血清可与原核表达的GST-FP25K融合蛋白及在感染的昆虫细胞中表达的FP25K蛋白发生特异性免疫反应。该抗体的获得为深入研究FP25K蛋白的功能提供了基础。
Keyword棉铃虫单核衣壳核多角体病毒 基因 克隆 表达 抗体 杆状病毒
Language英语
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/42257
Collection中科院水生所知识产出(2009年前)
Affiliation中国科学院水生生物研究所
Recommended Citation
GB/T 7714
吴东,邓菲,胡志红,等. 棉铃虫病毒HaSNPV fp25k基因的克隆表达及抗体制备[J]. 中国病毒学,2004,19.0(004):380.
APA 吴东,邓菲,胡志红,袁丽,&孙修炼.(2004).棉铃虫病毒HaSNPV fp25k基因的克隆表达及抗体制备.中国病毒学,19.0(004),380.
MLA 吴东,et al."棉铃虫病毒HaSNPV fp25k基因的克隆表达及抗体制备".中国病毒学 19.0.004(2004):380.
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