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SUPPRESSION OF LIGASE4 OR XRCC6 ACTIVITIES ENHANCES THE DNA HOMOLOGOUS RECOMBINATION EFFICIENCY IN ZEBRAFISH PRIMORDIAL GERM CELLS
Wei Zhiqiang; Xiong Feng; He Mudan; Wang Houpeng; Zhu Zuoyan; Sun Yonghua
2015
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume39Issue:2Pages:339
AbstractPrimordial germ cells (PGCs) give rise to gametes which transmit the genetic information to next generation, therefore PGCs provide us an ideal cell type for genetic manipulation. Homologous recombination (HR) is the most efficient technique to create designed genetic modifications, however, its efficiency is rather low in vertebrates. In this study, by using zebrafish as an in vivo model, we aimed to enhance the efficiency of HR in zebrafish PGCs. First, we injected UAS:mRFP-nos1 construct into Tg (kop:KalTA4) embryos to label the transgenic PGCs, and we showed that screening of PGCs-specific mRFP expression led to relatively high-efficient germline transmission of transgene. Then we established an in vivo assay to evaluate the HR frequency in PGCs. We further revealed that suppression of the activities of DNA ligase IV (Lig4) and Xrcc6 (previously known as Ku70) could significantly increase the HR efficiency, not only at whole embryo level but also in PGCs. We proposed that the Tg(kop:KalTA4) line could be used as an effective platform for HR-mediated gene targeting.
Keyword斑马鱼 原始生殖细胞 基因打靶 同源重组
Language英语
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/42085
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation中国科学院水生生物研究所
Recommended Citation
GB/T 7714
Wei Zhiqiang,Xiong Feng,He Mudan,et al. SUPPRESSION OF LIGASE4 OR XRCC6 ACTIVITIES ENHANCES THE DNA HOMOLOGOUS RECOMBINATION EFFICIENCY IN ZEBRAFISH PRIMORDIAL GERM CELLS[J]. Acta Hydrobiologica Sinica,2015,39(2):339.
APA Wei Zhiqiang,Xiong Feng,He Mudan,Wang Houpeng,Zhu Zuoyan,&Sun Yonghua.(2015).SUPPRESSION OF LIGASE4 OR XRCC6 ACTIVITIES ENHANCES THE DNA HOMOLOGOUS RECOMBINATION EFFICIENCY IN ZEBRAFISH PRIMORDIAL GERM CELLS.Acta Hydrobiologica Sinica,39(2),339.
MLA Wei Zhiqiang,et al."SUPPRESSION OF LIGASE4 OR XRCC6 ACTIVITIES ENHANCES THE DNA HOMOLOGOUS RECOMBINATION EFFICIENCY IN ZEBRAFISH PRIMORDIAL GERM CELLS".Acta Hydrobiologica Sinica 39.2(2015):339.
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