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Isolation of axenic cyanobacterium and the promoting effect of associated bacterium on axenic cyanobacterium
Gao, Suqin1; Kong, Yun2,3,4,5; Yu, Jing1; Miao, Lihong1; Ji, Lipeng2; Song, Lirong6; Zeng, Chi1
Corresponding AuthorMiao, Lihong(ky020241@hotmail.com)
2020-12-30
Source PublicationBMC BIOTECHNOLOGY
Volume20Issue:1Pages:11
AbstractBackground Harmful cyanobacterial blooms have attracted wide attention all over the world as they cause water quality deterioration and ecosystem health issues. Microcystis aeruginosa associated with a large number of bacteria is one of the most common and widespread bloom-forming cyanobacteria that secret toxins. These associated bacteria are considered to benefit from organic substrates released by the cyanobacterium. In order to avoid the influence of associated heterotrophic bacteria on the target cyanobacteria for physiological and molecular studies, it is urgent to obtain an axenic M. aeruginosa culture and further investigate the specific interaction between the heterotroph and the cyanobacterium. Results A traditional and reliable method based on solid-liquid alternate cultivation was carried out to purify the xenic cyanobacterium M. aeruginosa FACHB-905. On the basis of 16S rDNA gene sequences, two associated bacteria named strain B905-1 and strain B905-2, were identified as Pannonibacter sp. and Chryseobacterium sp. with a 99 and 97% similarity value, respectively. The axenic M. aeruginosa FACHB-905A (Microcystis 905A) was not able to form colonies on BG(11) agar medium without the addition of strain B905-1, while it grew well in BG(11) liquid medium. Although the presence of B905-1 was not indispensable for the growth of Microcystis 905A, B905-1 had a positive effect on promoting the growth of Microcystis 905A. Conclusions The associated bacteria were eliminated by solid-liquid alternate cultivation method and the axenic Microcystis 905A was successfully purified. The associated bacterium B905-1 has the potentiality to promote the growth of Microcystis 905A. Moreover, the purification technique for cyanobacteria described in this study is potentially applicable to a wider range of unicellular cyanobacteria.
KeywordMicrocystis aeruginosa Bacterial symbioses Microbial ecology Heterotrophic bacteria Promoting effect
DOI10.1186/s12896-020-00656-5
Funding OrganizationNational High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province
Indexed BySCI ; SCI
Language英语
Funding ProjectNational High Technology Research and Development Program of China (863 Program)[2013AA102805-04] ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province[2018ZJSHKF06] ; Key Project of Jingzhou Science and Technology[2019EC61-15] ; China Postdoctoral Science Foundation[2016 M591832] ; Natural Science Foundation of Jiangsu Province[BK20150165] ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province[KJ15ZB01]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000594796500001
WOS KeywordMICROCYSTIS-AERUGINOSA ; HETEROTROPHIC BACTERIA ; PHYLOGENETIC DIVERSITY ; GROWTH ; PURIFICATION ; CULTURE ; WATER ; SPP. ; ESTABLISHMENT ; AGGREGATION
PublisherBMC
Funding OrganizationNational High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; National High Technology Research and Development Program of China (863 Program) ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; Key Project of Jingzhou Science and Technology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Natural Science Foundation of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province ; Science and Technology Program of Administration of Quality and Technology Supervision of Jiangsu Province
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/39414
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorMiao, Lihong
Affiliation1.Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China
2.Yangtze Univ, Coll Resources & Environm, Wuhan 430100, Hubei, Peoples R China
3.Key Lab Water Pollut Control & Environm Safety Zh, Hangzhou 310058, Zhejiang, Peoples R China
4.Nanjing Univ, Yixing Acad Environm Protect, Yixing 214200, Jiangsu, Peoples R China
5.Natl Supervis & Inspect Ctr Environm Protect Equi, Yixing Urban Supervis & Inspect Adm Prod Qual, Yixing 214205, Jiangsu, Peoples R China
6.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Gao, Suqin,Kong, Yun,Yu, Jing,et al. Isolation of axenic cyanobacterium and the promoting effect of associated bacterium on axenic cyanobacterium[J]. BMC BIOTECHNOLOGY,2020,20(1):11.
APA Gao, Suqin.,Kong, Yun.,Yu, Jing.,Miao, Lihong.,Ji, Lipeng.,...&Zeng, Chi.(2020).Isolation of axenic cyanobacterium and the promoting effect of associated bacterium on axenic cyanobacterium.BMC BIOTECHNOLOGY,20(1),11.
MLA Gao, Suqin,et al."Isolation of axenic cyanobacterium and the promoting effect of associated bacterium on axenic cyanobacterium".BMC BIOTECHNOLOGY 20.1(2020):11.
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