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An identified PfHMGB1 promotes microcystin-LR-induced liver injury of yellow catfish (Pelteobagrus fulvidraco)
Wang, Yun1,2; Xiao, Xiaoxue2; Wang, Feijie2; Yang, Zupeng2; Yue, Jingkai2; Shi, Jiale2; Ke, Fei3; Xie, Zhaohui2; Fan, Yanru2
Corresponding AuthorWang, Yun(ywang401@jhun.edu.cn)
2021
Source PublicationECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN0147-6513
Volume207Pages:11
AbstractMicrocystin-LR (MC-LR) is a potent hepatotoxin that can cause liver inflammation and injury. However, the mode of action of related inflammatory factors is not fully understood. PfHMGB1 is an inflammatory factor induced at the mRNA level in the liver of juvenile yellow catfish (Pelteobagrus fulvidraco) that were intraperi-toneally injected with 50 mu g/kg MC-LR. The PfHMGB1 mRNA level was highest in the liver and muscle among 11 tissues examined. The full-length cDNA sequence of PfHMGB1 was cloned and overexpressed in E. coli, and the purified protein rPfHMGB1 demonstrated DNA binding affinity. Endotoxin-free rPfHMGB1 (6-150 mu g/mL) also showed dose-dependent hepatotoxicity and induced inflammatory gene expression of primary hepatocytes. PfHMGB1 antibody (anti-PfHMGB1) in vitro reduced MC-LR (30 and 50 mu mol/L)-induced hepatotoxicity, suggesting PfHMGB1 is important in the toxic effects of MC-LR. In vivo study showed that MC-LR upregulated PfHMGB1 protein in the liver. The anti-PfHMGB1 blocked its counterpart and reduced ALT/AST activities after MC-LR exposure. Anti-PfHMGB1 partly neutralized MC-LR-induced hepatocyte disorganization, nucleus shrinkage, mitochondria, and rough endoplasmic reticula destruction. These findings suggest that PfHMGB1 promotes MC-LR-induced liver damage in the yellow catfish. HMGB1 may help protect catfish against widespread microcystin pollution.
KeywordPfHMGB1 MC-LR Hepatotoxicity Liver injury Yellow catfish anti-PfHMGB1
DOI10.1016/j.ecoenv.2020.111266
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31702388]
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000592451000008
WOS KeywordGROUP BOX 1 ; INDUCED HEPATOCYTE APOPTOSIS ; DNA-BINDING ; TIGHT JUNCTIONS ; IN-VIVO ; HMGB1 ; EXPRESSION ; PROTEIN ; INFLAMMATION ; RESPONSES
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/39402
Collection其他_期刊论文
Corresponding AuthorWang, Yun
Affiliation1.Jianghan Univ, Inst Environm & Hlth, Hubei Key Lab Environm & Hlth Effects Persistent, Wuhan 430056, Hubei, Peoples R China
2.Henan Univ Urban Construct, Henan Prov Key Lab Water Pollut Control & Rehabil, Pingdingshan 467036, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yun,Xiao, Xiaoxue,Wang, Feijie,et al. An identified PfHMGB1 promotes microcystin-LR-induced liver injury of yellow catfish (Pelteobagrus fulvidraco)[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,207:11.
APA Wang, Yun.,Xiao, Xiaoxue.,Wang, Feijie.,Yang, Zupeng.,Yue, Jingkai.,...&Fan, Yanru.(2021).An identified PfHMGB1 promotes microcystin-LR-induced liver injury of yellow catfish (Pelteobagrus fulvidraco).ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,207,11.
MLA Wang, Yun,et al."An identified PfHMGB1 promotes microcystin-LR-induced liver injury of yellow catfish (Pelteobagrus fulvidraco)".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 207(2021):11.
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