| Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors | |
| He, Mudan1,2; Zhang, Ru1,2; Jiao, Shengbo1,2; Zhang, Fenghua1,2; Ye, Ding1,2; Wang, Houpeng1,2; Sun, Yonghua1,2 | |
| Corresponding Author | Sun, Yonghua(yhsun@ihb.ac.cn) |
| 2020-07-01 | |
| Source Publication | PLOS BIOLOGY
 |
| ISSN | 1544-9173 |
| Volume | 18Issue:7Pages:32 |
| Abstract | Maternal beta-catenin activity is essential and critical for dorsal induction and its dorsal activation has been thoroughly studied. However, how the maternal beta-catenin activity is suppressed in the nondorsal cells remains poorly understood. Nanog is known to play a central role for maintenance of the pluripotency and maternal -zygotic transition (MZT). Here, we reveal a novel role of Nanog as a strong repressor of maternal beta-catenin signaling to safeguard the embryo against hyperactivation of maternal beta-catenin activity and hyperdorsalization. In zebrafish, knockdown of nanog at different levels led to either posteriorization or dorsalization, mimicking zygotic or maternal activation of Wnt/beta-catenin activities, and the maternal zygotic mutant of nanog (MZnanog) showed strong activation of maternal beta-catenin activity and hyperdorsalization. Although a constitutive activator-type Nanog (Vp16-Nanog, lacking the N terminal) perfectly rescued the MZT defects of MZnanog, it did not rescue the phenotypes resulting from beta-catenin signaling activation. Mechanistically, the N terminal of Nanog directly interacts with T-cell factor (TCF) and interferes with the binding of beta-catenin to TCF, thereby attenuating the transcriptional activity of beta-catenin. Therefore, our study establishes a novel role for Nanog in repressing maternal beta-catenin activity and demonstrates a transcriptional switch between beta-catenin/TCF and Nanog/TCF complexes, which safeguards the embryo from global activation of maternal beta-catenin activity. |
| DOI | 10.1371/journal.pbio.3000561 |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31671501] ; National Natural Science Foundation of China[31702323] ; National key R&D Program of China[2018YFA0801000] ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology[2019FBZ05] |
| WOS Research Area | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
| WOS Subject | Biochemistry & Molecular Biology ; Biology |
| WOS ID | WOS:000596578100001 |
| WOS Keyword | WNT SIGNALING PATHWAY ; EMBRYONIC-DEVELOPMENT ; TRANSCRIPTION FACTORS ; DISTINCT ROLES ; AXIS FORMATION ; ZEBRAFISH ; ORGANIZER ; GROUCHO ; MESODERM ; GENES |
| Publisher | PUBLIC LIBRARY SCIENCE |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; National key R&D Program of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/39383 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Sun, Yonghua |
| Affiliation | 1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | He, Mudan,Zhang, Ru,Jiao, Shengbo,et al. Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors[J]. PLOS BIOLOGY,2020,18(7):32. |
| APA | He, Mudan.,Zhang, Ru.,Jiao, Shengbo.,Zhang, Fenghua.,Ye, Ding.,...&Sun, Yonghua.(2020).Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors.PLOS BIOLOGY,18(7),32. |
| MLA | He, Mudan,et al."Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors".PLOS BIOLOGY 18.7(2020):32. |
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