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Germination-Arrest Bacillus subtilis Spores as An Oral Delivery Vehicle of Grass Carp Reovirus (GCRV) Vp7 Antigen Augment Protective Immunity in Grass Carp (Ctenopharyngodon idella)
Sun, Rui1; Zhang, Miao1; Chen, Hui2; Wei, Yao1; Ning, Degang1,3
Corresponding AuthorNing, Degang(ningdegang@ihb.ac.cn)
2020-11-01
Source PublicationGENES
Volume11Issue:11Pages:15
AbstractOral vaccination is a practical method for the active immunization of farmed fish in the matter of animal welfare and handling costs. However, it always shows insufficient protective immunity, mainly due to antigen degradation in the gastrointestinal tract (GIT). Bacillus subtilis spores have been shown to be able to protect surface-display heterologous antigens against degradation. Neverthless, the spores can germinate in GIT, which causes loss of the antigens with spore coat disassembly. Here, we developed a novel surface display system using the B. subtilis spore coat proteins CotB and CotC as anchors for the heterogenous antigen, and the germination-controlling genes cwlJ and sleB as the ectopic integration sites for the fusion genes. Using this display system, we engineered germination-arrest spores displaying the model antigen Vp7 of grass carp reovirus (GCRV) on their surface. Oral vaccination of the engineered spores could confer immune protection against GCRV in grass carp (Ctenopharyngodon idella) via eliciting adaptive humoral and cellular immune responses. Most importantly, the germination-arrest spores were shown to significantly augment immunogenicity and protection above the engineered spores based on the existing surface display system. Therefore, the presently reported antigen expression strategy opens new and promising avenues for developing oral vaccines for the immunization of farmed fish species.
KeywordBacillus subtilis germination-arrest spore surface display GCRV oral vaccination protective immunity grass carp
DOI10.3390/genes11111351
Funding OrganizationKey Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian
Indexed BySCI ; SCI
Language英语
Funding ProjectKey Research and Development Project of Jiangsu[BE2015330] ; Agricultural Independent Innovation Project of Jiangsu[CX(17)2027] ; Key Research and Development Project of Huaian[HAN201811]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000593835200001
WOS KeywordTUMOR-NECROSIS-FACTOR ; PROTEIN ; SURFACE ; GENE ; REPERTOIRES ; INDUCTION ; VACCINES ; VIRUS
PublisherMDPI
Funding OrganizationKey Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Key Research and Development Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Agricultural Independent Innovation Project of Jiangsu ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian ; Key Research and Development Project of Huaian
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/39377
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorNing, Degang
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Huaian Res Ctr, Huaian 223005, Peoples R China
2.China Jiangsu Ctr Aquat Anim Dis Prevent & Contro, Nanjing 210036, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, CAS Key Lab Algal Biol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Sun, Rui,Zhang, Miao,Chen, Hui,et al. Germination-Arrest Bacillus subtilis Spores as An Oral Delivery Vehicle of Grass Carp Reovirus (GCRV) Vp7 Antigen Augment Protective Immunity in Grass Carp (Ctenopharyngodon idella)[J]. GENES,2020,11(11):15.
APA Sun, Rui,Zhang, Miao,Chen, Hui,Wei, Yao,&Ning, Degang.(2020).Germination-Arrest Bacillus subtilis Spores as An Oral Delivery Vehicle of Grass Carp Reovirus (GCRV) Vp7 Antigen Augment Protective Immunity in Grass Carp (Ctenopharyngodon idella).GENES,11(11),15.
MLA Sun, Rui,et al."Germination-Arrest Bacillus subtilis Spores as An Oral Delivery Vehicle of Grass Carp Reovirus (GCRV) Vp7 Antigen Augment Protective Immunity in Grass Carp (Ctenopharyngodon idella)".GENES 11.11(2020):15.
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