IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae
Zhang, Yu1; Pan, Yufang2; Ding, Wei1; Hu, Hanhua2; Liu, Jin1
Corresponding AuthorLiu, Jin(gjinliu@pku.edu.cn)
2020-11-10
Source PublicationGLOBAL CHANGE BIOLOGY BIOENERGY
ISSN1757-1693
Pages16
AbstractOleaginous algae have the ability to synthesize a high level of triacylglycerol (TAG) and are considered as the next-generation feedstock for biofuel production. Manipulating algal lipid biosynthetic pathways has potential to overproduce TAG and represents a feasible way toward bringing down algae-derived biofuels production cost. Here we dissected functional roles and engineering potential of six diacylglycerol acyltransferase (DGAT) genes from the marine alga Phaeodactylum tricornutum. PtDGAT1, PtDGAT2B, and PtDGAT3, residing at the chloroplast endoplasmic reticulum (ER) readily for utilizing both chloroplast- and ER-derived diacylglycerol, were transcriptionally correlated with TAG accumulation. Heterologous expression in yeast, in vitro assay and overexpression in P. tricornutum all supported that PtDGAT1 surpassed the other five PtDGATs in synthesizing TAG. Compared to wild type P. tricornutum, the PtDGAT1-overexpressing strain produced more than doubled TAG and total lipids, which reached 57% and 73% of dry weight, respectively, record-high levels ever achieved in this alga. Our results demonstrated the strategy of screening proper engineering targets and manipulating a single gene to pull carbon flux to lipids for TAG hyper-accumulation without growth compromise. The engineered alga with a great trait improvement may serve as a potent lipid producer for production uses.
Keywordbiofuel diacylglycerol acyltransferase genetic manipulation oleaginous alga Phaeodactylum tricornutum subcellular localization triacylglycerol
DOI10.1111/gcbb.12771
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31571807] ; National Natural Science Foundation of China[41976119] ; Peking University CCUS by BHP Billiton
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgronomy ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000587924700001
WOS KeywordFATTY-ACID BIOSYNTHESIS ; PHAEODACTYLUM-TRICORNUTUM ; CHLAMYDOMONAS-REINHARDTII ; TRIACYLGLYCEROL ACCUMULATION ; PHOSPHORUS STARVATION ; GREEN MICROALGA ; ACYL-COENZYME ; OIL SYNTHESIS ; IDENTIFICATION ; MEMBRANE
PublisherWILEY
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton ; Peking University CCUS by BHP Billiton
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/39290
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorLiu, Jin
Affiliation1.Peking Univ, Coll Engn, Lab Algae Biotechnol & Innovat, Beijing 100871, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yu,Pan, Yufang,Ding, Wei,et al. Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae[J]. GLOBAL CHANGE BIOLOGY BIOENERGY,2020:16.
APA Zhang, Yu,Pan, Yufang,Ding, Wei,Hu, Hanhua,&Liu, Jin.(2020).Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae.GLOBAL CHANGE BIOLOGY BIOENERGY,16.
MLA Zhang, Yu,et al."Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae".GLOBAL CHANGE BIOLOGY BIOENERGY (2020):16.
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