IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Cloning and functional characterization of IRAK1 from rainbow trout (Oncorhynchus mykiss)
Yue Cong Yang1,2,3; Shan Nan Chen1; Gan, Zhen1; Huang, Lin1; Nie, P.1,2,3,4,5
Corresponding AuthorNie, P.(pinnie@ihb.ac.cn)
2021
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume114Pages:8
AbstractAs a key molecule in innate immune signalling pathway, interleukin (IL)-1 receptor-associated kinase 1 (IRAK1) mediates downstream signalling cascades in immune response. In the present study, an IRAK1 orthologue was characterized from rainbow trout (Oncorhynchus mykiss), with a 2115 bp open reading frame (ORF), encoding a protein of 704 amino acids (aa). Multiple alignments showed that IRAK1 contains highly conserved features among different species, with a conservative N-terminal death domain (DD) and a C-terminal conserved serine/threonine protein kinase (STKc) domain. Expression analysis indicated that IRAK1 was widely expressed in examined organs/tissues, with the highest level observed in muscle and lowest in stomach. In RTG-2 cell line, the induced expression of IRAK1 was observed following the stimulation by the fish bacterial pathogen Flayobacterium columnare. Luciferase activity assays revealed that IRAK1 induced significantly the activity of NF-kappa B in Human embryonic kidney 293T (HEK293T) cell line; but after co-transfected with rainbow trout IL-1 receptorassociated kinase 4 (IRAK4), the induction was significantly down-regulated when compared with the expression of IRAK1 alone. Co-immunoprecipitation (Co-IP) assays indicated that IRAK1 was associated with rainbow trout myeloid differentiation factor 88 (MyD88), IRAK4 and TNF receptor associated factor 6 (TRAF6) in transfected HEK293T cells, and may form a complex with MyD88, IRAK4 and TRAF6 during the signalling pathway.
KeywordIRAK1 NF-kappa B MyD88 IRAK4 TRAF6 Rainbow trout
DOI10.1016/j.dci.2020.103780
Funding OrganizationNSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China
Indexed BySCI ; SCI
Language英语
Funding ProjectNSFC-Shandong Joint Fund[U1706205] ; China Agriculture Research System[CARS46] ; special top talent plan One Thing One Decision (Yishi Yiyi)[(2018)27] ; First Class Fishery Discipline Programme in Shandong Province, China[(2018)8]
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000588061100002
WOS KeywordRECEPTOR-ASSOCIATED KINASE ; INTERFERON REGULATORY FACTOR ; EXPRESSION ANALYSIS ; DEATH DOMAIN ; INTERLEUKIN-1 ; IDENTIFICATION ; GENE ; TOLLIP ; FISH ; THREONINE-66
PublisherELSEVIER SCI LTD
Funding OrganizationNSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; NSFC-Shandong Joint Fund ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; special top talent plan One Thing One Decision (Yishi Yiyi) ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China ; First Class Fishery Discipline Programme in Shandong Province, China
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/39263
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Innovat Acad Seed Design, Chinese Acad Sci, Wuhan, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
5.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Yue Cong Yang,Shan Nan Chen,Gan, Zhen,et al. Cloning and functional characterization of IRAK1 from rainbow trout (Oncorhynchus mykiss)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,114:8.
APA Yue Cong Yang,Shan Nan Chen,Gan, Zhen,Huang, Lin,&Nie, P..(2021).Cloning and functional characterization of IRAK1 from rainbow trout (Oncorhynchus mykiss).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,114,8.
MLA Yue Cong Yang,et al."Cloning and functional characterization of IRAK1 from rainbow trout (Oncorhynchus mykiss)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 114(2021):8.
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