| Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production | |
| Li, Tianpei1,2,3,4; Jiang, Qiuyao1; Huang, Jiafeng1,5; Aitchison, Catherine M.6,7; Huang, Fang1; Yang, Mengru; Dykes, Gregory F.1; He, Hai-Lun5; Wang, Qiang4,8; Sprick, Reiner Sebastian6,7; Cooper, Andrew I.6,7; Liu, Lu-Ning1,9,10 | |
| Corresponding Author | Wang, Qiang(wangqiang@henu.edu.cn) ; Liu, Lu-Ning(luning.liu@liverpool.ac.uk) |
| 2020-10-28 | |
| Source Publication | NATURE COMMUNICATIONS
 |
| ISSN | 2041-1723 |
| Volume | 11Issue:1Pages:10 |
| Abstract | Compartmentalization is a ubiquitous building principle in cells, which permits segregation of biological elements and reactions. The carboxysome is a specialized bacterial organelle that encapsulates enzymes into a virus-like protein shell and plays essential roles in photosynthetic carbon fixation. The naturally designed architecture, semi-permeability, and catalytic improvement of carboxysomes have inspired rational design and engineering of new nanomaterials to incorporate desired enzymes into the protein shell for enhanced catalytic performance. Here, we build large, intact carboxysome shells (over 90nm in diameter) in the industrial microorganism Escherichia coli by expressing a set of carboxysome protein-encoding genes. We develop strategies for enzyme activation, shell self-assembly, and cargo encapsulation to construct a robust nanoreactor that incorporates catalytically active [FeFe]-hydrogenases and functional partners within the empty shell for the production of hydrogen. We show that shell encapsulation and the internal microenvironment of the new catalyst facilitate hydrogen production of the encapsulated oxygen-sensitive hydrogenases. The study provides insights into the assembly and formation of carboxysomes and paves the way for engineering carboxysome shell-based nanoreactors to recruit specific enzymes for diverse catalytic reactions. The extreme oxygen sensitive character of hydrogenases is a longstanding issue for hydrogen production in bacteria. Here, the authors build carboxysome shells in E. coli and incorporate catalytically active hydrogenases and functional partners within the empty shell for the production of hydrogen. |
| DOI | 10.1038/s41467-020-19280-0 |
| Funding Organization | Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Royal Society University Research Fellowship[UF120411] ; Royal Society University Research Fellowship[URF\R\180030] ; Royal Society Fellow Enhancement Awards[RGF\EA\181061] ; Royal Society Fellow Enhancement Awards[RGF\EA\180233] ; Biotechnology and Biological Sciences Research Council[BB/M024202/1] ; Biotechnology and Biological Sciences Research Council[BB/R003890/1] ; British Council Newton Fund Grant[201703780114] ; British Council Newton Fund Grant[201806370307] ; Leverhulme Trust[ECF-2016-778] ; National Natural Science Foundation of China[91851103] ; National Natural Science Foundation of China[31770128] ; 111 Project[D16014] ; UK Engineering and Physical Sciences Research Council (EPSRC)[EP/N004884/1] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000588060400005 |
| WOS Keyword | HALOTHIOBACILLUS-NEAPOLITANUS CARBOXYSOMES ; CARBONIC-ANHYDRASE ; SHELL PROTEIN ; MICROCOMPARTMENTS ; ORGANIZATION ; PROCHLOROCOCCUS ; IDENTIFICATION ; EVOLUTION ; FIXATION ; REVEALS |
| Publisher | NATURE RESEARCH |
| Funding Organization | Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society University Research Fellowship ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Royal Society Fellow Enhancement Awards ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; Biotechnology and Biological Sciences Research Council ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; British Council Newton Fund Grant ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; Leverhulme Trust ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) ; UK Engineering and Physical Sciences Research Council (EPSRC) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/39260 |
| Collection | 藻类生物学及应用研究中心_期刊论文 |
| Corresponding Author | Wang, Qiang; Liu, Lu-Ning |
| Affiliation | 1.Univ Liverpool, Inst Syst Mol & Integrat Biol, Liverpool L69 7ZB, Merseyside, England 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Peoples R China 5.Cent South Univ, Sch Life Sci, Changsha, Peoples R China 6.Univ Liverpool, Mat Innovat Factory, Liverpool L7 3NY, Merseyside, England 7.Univ Liverpool, Dept Chem, Liverpool L7 3NY, Merseyside, England 8.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China 9.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 10.Ocean Univ China, Frontiers Sci Ctr Deep Ocean Multispheres & Earth, Qingdao 266003, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Tianpei,Jiang, Qiuyao,Huang, Jiafeng,et al. Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production[J]. NATURE COMMUNICATIONS,2020,11(1):10. |
| APA | Li, Tianpei.,Jiang, Qiuyao.,Huang, Jiafeng.,Aitchison, Catherine M..,Huang, Fang.,...&Liu, Lu-Ning.(2020).Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production.NATURE COMMUNICATIONS,11(1),10. |
| MLA | Li, Tianpei,et al."Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production".NATURE COMMUNICATIONS 11.1(2020):10. |
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