| Bioconcentration of 2,4,6-tribromophenol (TBP) and thyroid endocrine disruption in zebrafish larvae | |
Fu, Juanjuan1,2; Guo, Yongyong1; Wang, Min1; Yang, Lihua1; Han, Jian1 ; Lee, Jae-Seong3; Zhou, Bingsheng1
| |
| Corresponding Author | Han, Jian(hanjian@ihb.ac.cn) |
| 2020-12-15 | |
| Source Publication | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| ISSN | 0147-6513 |
| Volume | 206Pages:9 |
| Abstract | 2,4,6-tribromophenol (TBP) is generally used as a brominated flame retardant but is produced in the degradation of tetrabromobisphenol-A. Although TBP is frequently detected in the environment and in various biota, including fish species, we still know little about its toxicity and environmental health risk. Here we investigated the bioconcentration and effects of TBP on the thyroid endocrine system by using zebrafish as a model. Zebrafish embryos (2 h post-fertilization, hpf) were exposed to five concentrations of TBP (0, 0.3, 1, 10, and 100 mu g/L) until 144 hpf. According to our chemical analysis, TBP underwent bioconcentration in zebrafish larvae. However, acute exposure to TBP did not affect the hatching of embryos or their risk of malformation, nor the growth and survival of larvae, indicating low developmental toxicity of TBP. The whole-body thyroxine (T4) contents were significantly increased in zebrafish larvae after exposure to TBP, indicating thyroid endocrine disruption occurred. Gene transcription levels in the hypothalamic-pituitary-thyroid (HPT) axis were also examined in larvae; these results revealed that the transcription of corticotrophin-releasing hormone (crh), thyrotropin-releasing hormone (trh), and thyroid-stimulating hormone (tsh beta) were all significantly downregulated by exposure to TBP. Likewise, genes encoding thyronine deiodinases (dio1, dio2, and dio3a/b) and thyroid hormone receptors (tr alpha and tr beta) also had their transcription downregulated in zebrafish. Further, the gene transcription and protein expression of binding and transport protein transthyretin (TTR) were significantly increased after TBP exposure. Taken together, our results suggest the bioavailability of and potential thyroid endocrine disruption by TBP in fish. |
| Keyword | 2,4,6-tribromophenol Thyroid endocrine disruption Hypothalamic-pituitary-thyroid axis Thyroid hormones Brominated flame retardant Zebrafish larvae |
| DOI | 10.1016/j.ecoenv.2020.111207 |
| Funding Organization | National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Key Research and Development Program of China[2018YFD0900701] ; National Natural Science Foundation of China[21737005] ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances[PTS2019-04] |
| WOS Research Area | Environmental Sciences & Ecology ; Toxicology |
| WOS Subject | Environmental Sciences ; Toxicology |
| WOS ID | WOS:000582222400040 |
| WOS Keyword | POLYBROMINATED DIPHENYL ETHERS ; BROMINATED FLAME RETARDANTS ; DECABROMODIPHENYL ETHANE ; HORMONE RECEPTORS ; MESSENGER-RNAS ; HUMAN EXPOSURE ; BROMOPHENOLS ; EXPRESSION ; GROWTH ; PBDES |
| Publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| Funding Organization | National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances ; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/39222 |
| Collection | 水环境工程研究中心_期刊论文 |
| Corresponding Author | Han, Jian |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Sungkyunkwan Univ, Coll Sci, Dept Biol Sci, Suwon 16419, South Korea |
| Recommended Citation GB/T 7714 | Fu, Juanjuan,Guo, Yongyong,Wang, Min,et al. Bioconcentration of 2,4,6-tribromophenol (TBP) and thyroid endocrine disruption in zebrafish larvae[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2020,206:9. |
| APA | Fu, Juanjuan.,Guo, Yongyong.,Wang, Min.,Yang, Lihua.,Han, Jian.,...&Zhou, Bingsheng.(2020).Bioconcentration of 2,4,6-tribromophenol (TBP) and thyroid endocrine disruption in zebrafish larvae.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,206,9. |
| MLA | Fu, Juanjuan,et al."Bioconcentration of 2,4,6-tribromophenol (TBP) and thyroid endocrine disruption in zebrafish larvae".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 206(2020):9. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment