| Structural and Functional Insights into a Lysine Deacylase in the Cyanobacterium Synechococcus sp. PCC 7002 | |
| Liu, Xin1,2,3; Yang, Mingkun1,2; Liu, Yingfang4; Ge, Feng1,2,3; Zhao, Jindong1,5 | |
| Corresponding Author | Ge, Feng(gefeng@ihb.ac.cn) |
| 2020-10-01 | |
| Source Publication | PLANT PHYSIOLOGY
 |
| ISSN | 0032-0889 |
| Volume | 184Issue:2Pages:762-776 |
| Abstract | Lys deacylases are essential regulators of cell biology in many contexts. Here, we have identified CddA (cyanobacterial deacetylase/depropionylase), a Lys deacylase enzyme expressed in the cyanobacterium Synechococcus sp. PCC 7002 that has both deacetylase and depropionylase activity. Loss of the gene cddA led to slower growth and impaired linear and cyclic photosynthetic electron transfer. We determined the crystal structure of this depropionylase/deacetylase at 2.1 angstrom resolution and established that it has a unique and characteristically folded alpha/beta structure. We detected an acyl binding site within CddA via site-directed mutagenesis and demonstrated that this site is essential for the deproprionylase activity of this enzyme. Through a proteomic approach, we identified a total of 598 Lys residues across 382 proteins that were capable of undergoing propionylation. These propionylated proteins were highly enriched for photosynthetic and metabolic functionality. We additionally demonstrated that CddA was capable of catalyzing in vivo and in vitro Lys depropionylation and deacetylation of Fru-1,6-bisphosphatase, thereby regulating its enzymatic activity. Our identification of a Lys deacylase provides insight into the mechanisms globally regulating photosynthesis and carbon metabolism in cyanobacteria and potentially in other photosynthetic organisms as well. |
| DOI | 10.1104/pp.20.00583 |
| Funding Organization | National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Key Research and Development Program of China[2016YFA0501304] ; National Natural Science Foundation of China[31570829] ; Chinese Academy of Sciences[QYZDY-SSW-SMC004] ; Chinese Academy of Sciences Key Technology Talent Program |
| WOS Research Area | Plant Sciences |
| WOS Subject | Plant Sciences |
| WOS ID | WOS:000585831600029 |
| WOS Keyword | HISTONE DEACETYLASE HOMOLOG ; ACETYLOME ANALYSIS REVEALS ; CYCLIC ELECTRON FLOW ; PHOTOSYSTEM-I ; SP PCC-7002 ; POSTTRANSLATIONAL MODIFICATIONS ; MOLECULAR CHARACTERIZATION ; HYDROGEN-PEROXIDE ; ESCHERICHIA-COLI ; PROTEIN |
| Publisher | AMER SOC PLANT BIOLOGISTS |
| Funding Organization | National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program ; Chinese Academy of Sciences Key Technology Talent Program |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/39206 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Corresponding Author | Ge, Feng |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Sun Yat Sen Univ, Affiliated Hosp 6, Sch Med, Guangdong Prov Key Lab Colorectal & Pelv Floor Di, Guangzhou 510275, Guangdong, Peoples R China 5.Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing 100871, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Xin,Yang, Mingkun,Liu, Yingfang,et al. Structural and Functional Insights into a Lysine Deacylase in the Cyanobacterium Synechococcus sp. PCC 7002[J]. PLANT PHYSIOLOGY,2020,184(2):762-776. |
| APA | Liu, Xin,Yang, Mingkun,Liu, Yingfang,Ge, Feng,&Zhao, Jindong.(2020).Structural and Functional Insights into a Lysine Deacylase in the Cyanobacterium Synechococcus sp. PCC 7002.PLANT PHYSIOLOGY,184(2),762-776. |
| MLA | Liu, Xin,et al."Structural and Functional Insights into a Lysine Deacylase in the Cyanobacterium Synechococcus sp. PCC 7002".PLANT PHYSIOLOGY 184.2(2020):762-776. |
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