| Zebrafish Nedd8 facilitates ovarian development and the maintenance of female secondary sexual characteristics via suppression of androgen receptor activity | |
| Yu, Guangqing1,2; Liu, Xing1; Zhang, Dawei1; Wang, Jing1; Ouyang, Gang1; Chen, Zhu3; Xiao, Wuhan1,2,4,5,6 | |
| Corresponding Author | Xiao, Wuhan(w-xiao@ihb.ac.cn) |
| 2020-09-01 | |
| Source Publication | DEVELOPMENT
 |
| ISSN | 0950-1991 |
| Volume | 147Issue:18Pages:14 |
| Abstract | Nedd8 is a ubiquitin-like protein that covalently conjugates to target proteins through neddylation. In addition to cullin-RING ligases, neddylation also modifies non-cullin proteins to regulate protein activity, stability and localization. However, the roles of NEDD8 remain largely unknown in vivo. Here, we found that loss of nedd8 in female zebrafish led to defects in oogenesis, disrupted oocyte maturation and stimulated growth of the breeding tubercles (BTs) on the pectoral fins. The BTs are normally present in males, not females. However, the loss of one copy of ar can partially rescue the phenotypes displayed by nedd8-null female zebrafish. Further assays indicated that Nedd8 conjugates to Ar and Ar is neddylated at lysine 475 and lysine 862. Moreover, Nedd8 conjugation efficiently suppressed Ar transcriptional activity. Lysine 862 (K862) of Ar is the key site modified by neddylation to modulate Ar transcriptional activity. Thus, our results not only demonstrated that Nedd8 modulates ovarian maturation and the maintenance of female secondary sexual characteristics of female zebrafish in vivo, but also indicated that androgen signaling is strictly regulated by nedd8. |
| Keyword | Nedd8 Zebrafish Androgen receptor Ovary Testis Neddylation |
| DOI | 10.1242/dev.194886 |
| Funding Organization | Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24010308] ; National Natural Science Foundation of China[31830101] ; National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31671315] ; National Natural Science Foundation of China[31701074] ; National Key Research and Development Program of China Stem Cell and Translational Research[2018YFD0900602] |
| WOS Research Area | Developmental Biology |
| WOS Subject | Developmental Biology |
| WOS ID | WOS:000576692300019 |
| WOS Keyword | GERM-CELL DIFFERENTIATION ; POSTTRANSLATIONAL MODIFICATION ; PROTEIN NEDDYLATION ; SELF-RENEWAL ; PROSTATE ; REGENERATION ; EXPRESSION ; CANCER ; TESTES ; DEN1 |
| Publisher | COMPANY BIOLOGISTS LTD |
| Funding Organization | Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research ; National Key Research and Development Program of China Stem Cell and Translational Research |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/38956 |
| Collection | 水环境工程研究中心_期刊论文 |
| Corresponding Author | Xiao, Wuhan |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Dept Fisheries, Beijing 100049, Peoples R China 3.Maternal & Child Hlth Hosp Hubei Prov, Dept Reprod, Wuhan 430070, Peoples R China 4.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 6.Chinese Acad Sci, Innovat Acad Seed Design, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Yu, Guangqing,Liu, Xing,Zhang, Dawei,et al. Zebrafish Nedd8 facilitates ovarian development and the maintenance of female secondary sexual characteristics via suppression of androgen receptor activity[J]. DEVELOPMENT,2020,147(18):14. |
| APA | Yu, Guangqing.,Liu, Xing.,Zhang, Dawei.,Wang, Jing.,Ouyang, Gang.,...&Xiao, Wuhan.(2020).Zebrafish Nedd8 facilitates ovarian development and the maintenance of female secondary sexual characteristics via suppression of androgen receptor activity.DEVELOPMENT,147(18),14. |
| MLA | Yu, Guangqing,et al."Zebrafish Nedd8 facilitates ovarian development and the maintenance of female secondary sexual characteristics via suppression of androgen receptor activity".DEVELOPMENT 147.18(2020):14. |
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