IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Glutathione peroxidase 5 deficiency induces lipid metabolism regulated by reactive oxygen species in Chlamydomonas reinhardtii
Ma, Xiaocui1,2; Wei, Haiyan1,2; Zhang, Yaodong1,2; Duan, You3,4; Zhang, Wanting3; Cheng, Yingyin3; Xia, Xiao-Qin3,4; Shi, Mijuan3
Corresponding AuthorWei, Haiyan(haiyanwei2009@163.com) ; Shi, Mijuan(shimijuan@ihb.ac.cn)
2020-10-01
Source PublicationMICROBIAL PATHOGENESIS
ISSN0882-4010
Volume147Pages:5
AbstractBackground: Reactive oxygen species (ROS) are generated incidentally during natural metabolism process of aerobic photosynthetic organisms which could be either harmful for cellular components. How ROS regulated lipid metabolism and the transcriptomes of stressed cells respond to ROS in aerobic photosynthetic organisms are unclear. Glutathione peroxidases (GPXs) detoxify hydrogen peroxide or organic hydroperoxides, which are important enzymes of the antioxidant system. So the function of GPXs matters the cellular redox state. How the lipid metabolism respond to the GPXs deficiency remains to be explored. Methods: In this study, we employed a Chlamydomonas reinhardtii gpx5 knockout mutant to examine the effects of ROS on lipid metabolism. The redox state and lipid content of the parental strain CC4348 and the gpx5 mutant were detected. Besides, the transcriptomes of CC4348 and the gpx5 mutant were sequenced before and after treatment with nitrogen-free medium to obtain genome wide respond. Then we performed the functional annotation, classification and enrichment analysis based on KEGG database for the differentially expressed genes (DEGs) before and after nitrogen deprivation of CC4348 and the gpx5 mutant. Results: In the CC4348 cells, the lipid accumulated accompanying with increasing ROS level after treatment with nitrogen-free media. However, in the gpx5 mutant, the ROS level is much higher than that in the parental strain CC4348, unexpectedly with reduced lipid accumulation. By comparing the transcriptomes of CC4348 and gpx5 mutant, we found that both CC4348 and gpx5 mutant cells displayed upregulation of transcripts related to protein, nucleic acid, carbon metabolism and chlorophyll biosynthesis, but more proportion of genes related to lipid metabolism were up-regulated in CC4348 than that in the gpx5 mutant. Conclusion: In CC4348, lipid metabolism was up-regulated with increasing ROS level. But in the gpx5 mutant, Lipid accumulation was less with higher ROS level, which was due to the inhibited lipid biosynthesis. Therefore, ROS provides dual-directional regulation of lipid metabolism induced by GPX5 in Chlamydomonas.
KeywordGlutathione peroxidase Reactive oxygen species Lipid Transcriptome Chlamydomonas reinhardtii
DOI10.1016/j.micpath.2020.104358
Funding OrganizationWuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China
Indexed BySCI ; SCI
Language英语
Funding ProjectWuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China
WOS Research AreaImmunology ; Microbiology
WOS SubjectImmunology ; Microbiology
WOS IDWOS:000574906100008
WOS KeywordOXIDATIVE STRESS ; GENE
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationWuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38925
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorWei, Haiyan; Shi, Mijuan
Affiliation1.Zhengzhou Univ, Zhengzhou Childrens Hosp, Childrens Hosp, Henan Childrens Hosp, Zhengzhou 450000, Peoples R China
2.Zhengzhou Key Lab Pediat Endocrine Genet & Metab, Zhengzhou 450000, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Ma, Xiaocui,Wei, Haiyan,Zhang, Yaodong,et al. Glutathione peroxidase 5 deficiency induces lipid metabolism regulated by reactive oxygen species in Chlamydomonas reinhardtii[J]. MICROBIAL PATHOGENESIS,2020,147:5.
APA Ma, Xiaocui.,Wei, Haiyan.,Zhang, Yaodong.,Duan, You.,Zhang, Wanting.,...&Shi, Mijuan.(2020).Glutathione peroxidase 5 deficiency induces lipid metabolism regulated by reactive oxygen species in Chlamydomonas reinhardtii.MICROBIAL PATHOGENESIS,147,5.
MLA Ma, Xiaocui,et al."Glutathione peroxidase 5 deficiency induces lipid metabolism regulated by reactive oxygen species in Chlamydomonas reinhardtii".MICROBIAL PATHOGENESIS 147(2020):5.
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