Phylogenomic analysis of Balantidium ctenopharyngodoni (Ciliophora, Litostomatea) based on single-cell transcriptome sequencing
Sun, Zongyi1; Jiang, Chuanqi2; Feng, Jinmei3; Yang, Wentao2; Li, Ming1,2; Miao, Wei2
2017-11-14
Source PublicationPARASITE
ISSN1252-607X
Volume24
AbstractIn this paper, we present transcriptome data for Balantidium ctenopharyngodoni Chen, 1955 collected from the hindgut of grass carp (Ctenopharyngodon idella). We evaluated sequence quality and de novo assembled a preliminary transcriptome, including 43.3 megabits and 119,141 transcripts. Then we obtained a final transcriptome, including 17.7 megabits and 35,560 transcripts, by removing contaminative and redundant sequences. Phylogenomic analysis based on a supermatrix with 132 genes comprising 53,873 amino acid residues and phylogenetic analysis based on SSU rDNA of 27 species were carried out herein to reveal the evolutionary relationships among six ciliate groups: Colpodea, Oligohymenophorea, Litostomatea, Spirotrichea, Heterotrichea and Protocruziida. The topologies of both phylogenomic and phylogenetic trees are discussed in this paper. In addition, our results suggest that single-cell sequencing is a sound method of obtaining sufficient omics data for phylogenomic analysis, which is a good choice for uncultivable ciliates. The transcriptome data for Balantidium ctenopharyngodoni are the first omics data within the subclass Trichostomatia, and provide a good basis for ciliate phylogenomic analysis, as well as related omics analysis.
SubtypeArticle
KeywordBalantidium Ctenopharyngodoni Trichostomatia Single-cell Transcriptome Sequencing Ssu Rdna Phylogenomic Analysis
DOI10.1051/parasite/2017043
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNatural Science Foundation of China(31372168 ; Natural Science Foundation of China(31372168 ; 31471978 ; 31471978 ; 31501854) ; 31501854) ; Natural Science Foundation of China(31372168 ; Natural Science Foundation of China(31372168 ; 31471978 ; 31471978 ; 31501854) ; 31501854)
Language英语
WOS Research AreaParasitology
WOS SubjectParasitology
WOS KeywordRNA GENE-SEQUENCES ; SUBCLASS PERITRICHIA OLIGOHYMENOPHOREA ; EVOLUTIONARY RELATIONSHIPS ; SISTER GROUP ; GENOME ; CHEN ; PROTOCRUZIA ; POSITION ; CHINA ; PHYLOGENETICS
WOS IDWOS:000415038300001
Funding OrganizationNatural Science Foundation of China(31372168 ; Natural Science Foundation of China(31372168 ; 31471978 ; 31471978 ; 31501854) ; 31501854) ; Natural Science Foundation of China(31372168 ; Natural Science Foundation of China(31372168 ; 31471978 ; 31471978 ; 31501854) ; 31501854)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38896
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China
3.Jianghan Univ, Sch Med, Dept Pathogen Biol, Wuhan 430056, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Sun, Zongyi,Jiang, Chuanqi,Feng, Jinmei,et al. Phylogenomic analysis of Balantidium ctenopharyngodoni (Ciliophora, Litostomatea) based on single-cell transcriptome sequencing[J]. PARASITE,2017,24.
APA Sun, Zongyi,Jiang, Chuanqi,Feng, Jinmei,Yang, Wentao,Li, Ming,&Miao, Wei.(2017).Phylogenomic analysis of Balantidium ctenopharyngodoni (Ciliophora, Litostomatea) based on single-cell transcriptome sequencing.PARASITE,24.
MLA Sun, Zongyi,et al."Phylogenomic analysis of Balantidium ctenopharyngodoni (Ciliophora, Litostomatea) based on single-cell transcriptome sequencing".PARASITE 24(2017).
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