Structure of phycobilisome from the red alga Griffithsia pacifica
Zhang, Jun1,5; Ma, Jianfei1; Liu, Desheng1; Qin, Song2; Sun, Shan1; Zhao, Jindong3,4; Sui, Sen-Fang1
2017-11-02
Source PublicationNATURE
ISSN0028-0836
Volume551Issue:7678Pages:57-+
AbstractLife on Earth depends on photosynthesis for its conversion of solar energy to chemical energy. Photosynthetic organisms have developed a variety of light-harvesting systems to capture sunlight. The largest light-harvesting complex is the phycobilisome (PBS), the main light-harvesting antenna in cyanobacteria and red algae. It is composed of phycobiliproteins and linker proteins but the assembly mechanisms and energy transfer pathways of the PBS are not well understood. Here we report the structure of a 16.8-megadalton PBS from a red alga at 3.5 angstrom resolution obtained by single-particle cryo-electron microscopy. We modelled 862 protein subunits, including 4 linkers in the core, 16 rod-core linkers and 52 rod linkers, and located a total of 2,048 chromophores. This structure reveals the mechanisms underlying specific interactions between linkers and phycobiliproteins, and the formation of linker skeletons. These results provide a firm structural basis for our understanding of complex assembly and the mechanisms of energy transfer within the PBS.
SubtypeArticle
DOI10.1038/nature24278
WOS HeadingsScience & Technology
Indexed BySCI
Funding OrganizationNational Basic Research Program(2016YFA0501101 ; National Basic Research Program(2016YFA0501101 ; National Natural Science Foundation of China(31230016 ; National Natural Science Foundation of China(31230016 ; CAS fund(QYZDY-SSW-SMC004) ; CAS fund(QYZDY-SSW-SMC004) ; 2017YFA0504600 ; 2017YFA0504600 ; 31370717 ; 31370717 ; 2015CB150100) ; 2015CB150100) ; 31670745 ; 31670745 ; 31670746 ; 31670746 ; 31400632) ; 31400632) ; National Basic Research Program(2016YFA0501101 ; National Basic Research Program(2016YFA0501101 ; National Natural Science Foundation of China(31230016 ; National Natural Science Foundation of China(31230016 ; CAS fund(QYZDY-SSW-SMC004) ; CAS fund(QYZDY-SSW-SMC004) ; 2017YFA0504600 ; 2017YFA0504600 ; 31370717 ; 31370717 ; 2015CB150100) ; 2015CB150100) ; 31670745 ; 31670745 ; 31670746 ; 31670746 ; 31400632) ; 31400632)
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordEM STRUCTURE DETERMINATION ; TERMINAL ENERGY ACCEPTOR ; LIGHT-HARVESTING PIGMENT ; BILIN ATTACHMENT SITES ; ELECTRON-MICROSCOPY ; CRYO-EM ; CYANOBACTERIAL PHYCOBILISOME ; PORPHYRIDIUM-CRUENTUM ; ALLOPHYCOCYANIN B ; CRYSTAL-STRUCTURE
WOS IDWOS:000414222900043
Funding OrganizationNational Basic Research Program(2016YFA0501101 ; National Basic Research Program(2016YFA0501101 ; National Natural Science Foundation of China(31230016 ; National Natural Science Foundation of China(31230016 ; CAS fund(QYZDY-SSW-SMC004) ; CAS fund(QYZDY-SSW-SMC004) ; 2017YFA0504600 ; 2017YFA0504600 ; 31370717 ; 31370717 ; 2015CB150100) ; 2015CB150100) ; 31670745 ; 31670745 ; 31670746 ; 31670746 ; 31400632) ; 31400632) ; National Basic Research Program(2016YFA0501101 ; National Basic Research Program(2016YFA0501101 ; National Natural Science Foundation of China(31230016 ; National Natural Science Foundation of China(31230016 ; CAS fund(QYZDY-SSW-SMC004) ; CAS fund(QYZDY-SSW-SMC004) ; 2017YFA0504600 ; 2017YFA0504600 ; 31370717 ; 31370717 ; 2015CB150100) ; 2015CB150100) ; 31670745 ; 31670745 ; 31670746 ; 31670746 ; 31400632) ; 31400632)
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38895
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Tsinghua Univ, State Key Lab Membrane Biol, Beijing Adv Innovat Ctr Struct Biol, Sch Life Sci, Beijing 100084, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coast Zone Res, Yantai 264003, Peoples R China
3.Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing 100871, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Phycol CAS, Wuhan 430072, Hubei, Peoples R China
5.MRC Lab Mol Biol, Cambridge CB2 0QH, England
Recommended Citation
GB/T 7714
Zhang, Jun,Ma, Jianfei,Liu, Desheng,et al. Structure of phycobilisome from the red alga Griffithsia pacifica[J]. NATURE,2017,551(7678):57-+.
APA Zhang, Jun.,Ma, Jianfei.,Liu, Desheng.,Qin, Song.,Sun, Shan.,...&Sui, Sen-Fang.(2017).Structure of phycobilisome from the red alga Griffithsia pacifica.NATURE,551(7678),57-+.
MLA Zhang, Jun,et al."Structure of phycobilisome from the red alga Griffithsia pacifica".NATURE 551.7678(2017):57-+.
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