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题名: The Type IX Secretion System Is Required for Virulence of the Fish Pathogen Flavobacterium columnare
作者: Li, Nan1, 2; Zhu, Yongtao2; LaFrentz, Benjamin R.3; Evenhuis, Jason P.4; Hunnicutt, David W.5; Conrad, Rachel A.2; Barbier, Paul2; Gullstrand, Connor W.5; Roets, Jack E.5; Powers, Jonathan L.5; Kulkarni, Surashree S.2; Erbes, Devon H.2; Garcia, Julio C.3; Nie, Pin1; McBride, Mark J.2
关键词: columnaris disease ; gliding motility ; type IX secretion system ; virulence
刊名: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
发表日期: 2017-12-01
DOI: 10.1128/AEM.01769-17
卷: 83, 期:23
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Microbiology
英文摘要: Flavobacterium columnare, a member of the phylum Bacteroidetes, causes columnaris disease in wild and aquaculture-reared freshwater fish. The mechanisms responsible for columnaris disease are not known. Many members of the phylum Bacteroidetes use type IX secretion systems (T9SSs) to secrete enzymes, adhesins, and proteins involved in gliding motility. The F. columnare genome has all of the genes needed to encode a T9SS. gldN, which encodes a core component of the T9SS, was deleted in wild-type strains of F. columnare. The F. columnare Delta gldN mutants were deficient in the secretion of several extracellular proteins and lacked gliding motility. The Delta gldN mutants exhibited reduced virulence in zebrafish, channel catfish, and rainbow trout, and complementation restored virulence. PorV is required for the secretion of a subset of proteins targeted to the T9SS. An F. columnare Delta porV mutant retained gliding motility but exhibited reduced virulence. Cell-free spent media from exponentially growing cultures of wild-type and complemented strains caused rapid mortality, but spent media from Delta gldN and Delta porV mutants did not, suggesting that soluble toxins are secreted by the T9SS.
关键词[WOS]: TROUT ONCORHYNCHUS-MYKISS ; GRAM-NEGATIVE BACTERIA ; CATFISH ICTALURUS-PUNCTATUS ; C-TERMINAL-DOMAIN ; AC LYASE ACTIVITY ; GLIDING MOTILITY ; PORPHYROMONAS-GINGIVALIS ; PROTEIN SECRETION ; CHANNEL CATFISH ; FLEXIBACTER-COLUMNARIS
语种: 英语
项目资助者: USDA-ARS (CRIS)(6010-32000-026-00D) ; USDA-ARS(5090-31320-004-03S) ; National Science Foundation(MCB-1516990) ; Chinese Academy of Sciences(XDA08010207) ; Chinese Academy of Sciences ; University of Wisconsin Sea Grant Institute from National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce ; State of Wisconsin, federal grant(NA14OAR4170092 ; R/SFA-08 ; R/SFA-11)
WOS记录号: WOS:000415617800021
ISSN号: 0099-2240
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/38892
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Univ Wisconsin Milwaukee, Dept Biol Sci, Milwaukee, WI 53211 USA
3.USDA ARS, Aquat Anim Hlth Res Unit, Auburn, AL USA
4.USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV USA
5.St Norbert Coll, Dept Biol, De Pere, WI 54115 USA
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