| Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila | |
| Xiong, Fan1; Xiong, Jing2; Wu, Ya Fang2; Cao, Lu1,4; Huang, Wen Shu2; Chang, Ming Xian1,3,4 | |
| Corresponding Author | Huang, Wen Shu(wshuang@jmu.edu.cn) ; Chang, Ming Xian(mingxianchang@ihb.ac.cn) |
| 2020-10-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| Volume | 105Pages:297-309 |
| Abstract | No studies systematically examined the intestinal immune response for yellow stage of European eel (Anguilla anguilla) with Aeromonas hydrophila infection by time-resolved RNA-seq. Here, we examined transcriptional profiles of the intestines at three-time points following infection with A. hydrophila. Intraperitoneal injections caused mortalities within 48 h post-injection (hpi), with the survival rate 87.5% at 24 hpi and 83.9% at 48 hpi. The result from KEGG pathway enrichment analysis showed that the immune related "cytosolic DNA-sensing pathway" was significantly enriched at the first and second time points (6 hpi and 18 hpi), with the upregulated expression of irf3, ill b, tnfaip3, cxcl8a, ap1-2, c-fos, polr3d, polr3g and polr3k both at 6 hpi and 18 hpi, but not at the third time point (36 hpi). According to the KEGG annotation, 326 immune and inflammation-related DEGs were found. The co-expression network of those 326 DEGs revealed the existence of three modules, and tlrl was found to be in the center of the biggest module which contained massive DEGs from "signal transduction" and "transport and catabolism". The c3 isoforms showed different expression pattern among the three time points, indicating a unique activation of complement systems at 18 hpi. Furthermore, two cathelicidins (aaCATH_1 and aaCATH_2) were highly up-regulated at the first two time points, and the bacterial growth inhibition assay revealed their antibacterial properties against A. hydrophila. Our data indicated the important roles of cytosolic DNA-sensing pathway, as well as transcripts including tlr1, c3, polr and cathelicidins in the intestine of A. anguilla in response to A. hydrophila infection. The present study will provide leads for functional studies of host-pathogen interactions. |
| Keyword | Transcriptome analysis European eel Aeromonas hydrophila Cytosolic DNA-Sensing pathway Cathelicidins Intestine |
| DOI | 10.1016/j.fsi.2020.06.059 |
| Funding Organization | Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Natural Science Foundation of Fujian Province[2018J01452] ; National Natural Science Foundation of China[31672687] ; National Natural Science Foundation of China[31873046] |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000571806800004 |
| WOS Keyword | TOLL-LIKE RECEPTORS ; TROUT ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; TRANSCRIPTOME ANALYSIS ; NUCLEAR IMPORT ; REVEALS ; SPLEEN ; COMMON ; SIGNATURES ; BACTERIA |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Funding Organization | Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/38876 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Huang, Wen Shu; Chang, Ming Xian |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China 2.Jimei Univ, Engn Res Ctr Modern Technol Eel Ind, Minist Educ PR China, Xiamen 361021, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Xiong, Fan,Xiong, Jing,Wu, Ya Fang,et al. Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila[J]. FISH & SHELLFISH IMMUNOLOGY,2020,105:297-309. |
| APA | Xiong, Fan,Xiong, Jing,Wu, Ya Fang,Cao, Lu,Huang, Wen Shu,&Chang, Ming Xian.(2020).Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila.FISH & SHELLFISH IMMUNOLOGY,105,297-309. |
| MLA | Xiong, Fan,et al."Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila".FISH & SHELLFISH IMMUNOLOGY 105(2020):297-309. |
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