IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila
Xiong, Fan1; Xiong, Jing2; Wu, Ya Fang2; Cao, Lu1,4; Huang, Wen Shu2; Chang, Ming Xian1,3,4
Corresponding AuthorHuang, Wen Shu(wshuang@jmu.edu.cn) ; Chang, Ming Xian(mingxianchang@ihb.ac.cn)
2020-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume105Pages:297-309
AbstractNo studies systematically examined the intestinal immune response for yellow stage of European eel (Anguilla anguilla) with Aeromonas hydrophila infection by time-resolved RNA-seq. Here, we examined transcriptional profiles of the intestines at three-time points following infection with A. hydrophila. Intraperitoneal injections caused mortalities within 48 h post-injection (hpi), with the survival rate 87.5% at 24 hpi and 83.9% at 48 hpi. The result from KEGG pathway enrichment analysis showed that the immune related "cytosolic DNA-sensing pathway" was significantly enriched at the first and second time points (6 hpi and 18 hpi), with the upregulated expression of irf3, ill b, tnfaip3, cxcl8a, ap1-2, c-fos, polr3d, polr3g and polr3k both at 6 hpi and 18 hpi, but not at the third time point (36 hpi). According to the KEGG annotation, 326 immune and inflammation-related DEGs were found. The co-expression network of those 326 DEGs revealed the existence of three modules, and tlrl was found to be in the center of the biggest module which contained massive DEGs from "signal transduction" and "transport and catabolism". The c3 isoforms showed different expression pattern among the three time points, indicating a unique activation of complement systems at 18 hpi. Furthermore, two cathelicidins (aaCATH_1 and aaCATH_2) were highly up-regulated at the first two time points, and the bacterial growth inhibition assay revealed their antibacterial properties against A. hydrophila. Our data indicated the important roles of cytosolic DNA-sensing pathway, as well as transcripts including tlr1, c3, polr and cathelicidins in the intestine of A. anguilla in response to A. hydrophila infection. The present study will provide leads for functional studies of host-pathogen interactions.
KeywordTranscriptome analysis European eel Aeromonas hydrophila Cytosolic DNA-Sensing pathway Cathelicidins Intestine
DOI10.1016/j.fsi.2020.06.059
Funding OrganizationNatural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNatural Science Foundation of Fujian Province[2018J01452] ; National Natural Science Foundation of China[31672687] ; National Natural Science Foundation of China[31873046]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000571806800004
WOS KeywordTOLL-LIKE RECEPTORS ; TROUT ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; TRANSCRIPTOME ANALYSIS ; NUCLEAR IMPORT ; REVEALS ; SPLEEN ; COMMON ; SIGNATURES ; BACTERIA
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationNatural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; Natural Science Foundation of Fujian Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38876
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHuang, Wen Shu; Chang, Ming Xian
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China
2.Jimei Univ, Engn Res Ctr Modern Technol Eel Ind, Minist Educ PR China, Xiamen 361021, Peoples R China
3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Xiong, Fan,Xiong, Jing,Wu, Ya Fang,et al. Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila[J]. FISH & SHELLFISH IMMUNOLOGY,2020,105:297-309.
APA Xiong, Fan,Xiong, Jing,Wu, Ya Fang,Cao, Lu,Huang, Wen Shu,&Chang, Ming Xian.(2020).Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila.FISH & SHELLFISH IMMUNOLOGY,105,297-309.
MLA Xiong, Fan,et al."Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila".FISH & SHELLFISH IMMUNOLOGY 105(2020):297-309.
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