IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Molecular identification and function characterization of four finTRIM genes from the immortal fish cell line, EPC
Li, Yi-Lin1,2; Zhao, Xiang1,2; Gong, Xiu-Ying1,2; Dan, Cheng1,2; Gui, Jian-Fang1,2,3; Zhang, Yi-Bing1,2,3,4
Corresponding AuthorZhang, Yi-Bing(ybzhang@ihb.ac.cn)
2020-12-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume113Pages:11
AbstractIn mammals, tripartite motif (TRIM)-containing proteins are involved in interferon (IFN)-mediated antiviral response as pivotal players endowed with antiviral effects and modulatory capacity. Teleost fish have a unique subfamily of TRIM, called finTRIM (fish novel TRIM, FTR) generated by genus- or species-specific duplication of TRIM genes. Herein, four TRIM genes are identified from Epithelioma papulosum cyprini (EPC) cells, and phylogenetically close to the members of finTRIM, thus named FTREPC1, FTREPC2, FTREPC3 and FTREPC4. Despite high similarity in nucleotide sequence, FTREPC1/2 genes encode two proteins with a typically consecutive tripartite motif followed by a C-terminal B30.2 domain, while FTREPC3/4-encoding proteins retain only a RING domain due to early termination of translation. They are induced by poly(I:C), GCRV and SVCV as IFN-stimulated genes (ISGs), and this induction is severely impaired by blockade of STAT1 pathway and is dependent on a typical ISRE motif within the 5' untranslated regions (5'UTRs) of FTREPC1/2/3/4 genes. Whereas overexpression of FTREPC1/2/3/4 alone does not activate fish IFN promoters, overexpression of FTREPC1 or FTREPC2, rather than FTREPC3 and FTREPC4, significantly impairs intracellular poly(I:C)-triggered activation of fish IFN promoters. Consistently, FTREPC1/2 promote virus replication through negatively regulating IFN response. Our results provide evidence for the involvement of EPC finTRIM proteins in IFN antiviral response and insights into genus- or species-specific regulation of fish innate immune pathways.
KeywordfinTRIM ISGs Negative regulation IFN antiviral Response
DOI10.1016/j.dci.2020.103775
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory
Indexed BySCI ; SCI
Language英语
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA24010308] ; National Key R&D Program of China[2018YFD0900302] ; National Natural Science Foundation[31772875] ; National Natural Science Foundation[31972826] ; Freshwater Ecology and Biotechnology Laboratory[2019FBZ04]
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000571143400002
WOS KeywordANTIVIRAL IMMUNE-RESPONSE ; NEGATIVE REGULATION ; FAMILY ; EXERTS ; PROTEIN ; UBIQUITINATION ; IRIDOVIRUS ; PATHWAY ; TRIM13 ; ROLES
PublisherELSEVIER SCI LTD
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38863
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, Yi-Bing
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
3.Chinese Acad Sci, Innovat Acad Seed Design, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, Yi-Lin,Zhao, Xiang,Gong, Xiu-Ying,et al. Molecular identification and function characterization of four finTRIM genes from the immortal fish cell line, EPC[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,113:11.
APA Li, Yi-Lin,Zhao, Xiang,Gong, Xiu-Ying,Dan, Cheng,Gui, Jian-Fang,&Zhang, Yi-Bing.(2020).Molecular identification and function characterization of four finTRIM genes from the immortal fish cell line, EPC.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,113,11.
MLA Li, Yi-Lin,et al."Molecular identification and function characterization of four finTRIM genes from the immortal fish cell line, EPC".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 113(2020):11.
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