IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species
He, Xiaozhen1; Chen, Wenfeng1; Liu, Zhen2; Yu, Guirong1; Chen, Youbang1; Cai, Yi-Jun2; Sun, Ling1; Xu, Wanli1; Zhong, Lili1; Gao, Caixi1; Chen, Jishen1; Zhang, Minjie1; Yang, Shengxi1; Yao, Yizhou1; Zhang, Zhiping1; Ma, Fujun1; Zhang, Chen-Chen2; Lu, Hui-Ping2; Yu, Bin2; Cheng, Tian-Lin2; Qiu, Juhui3; Sheng, Qing4; Zhou, Hai-Meng4,5; Lv, Zhi-Rong5; Yan, Junjun6; Zhou, Yongjian7; Qiu, Zilong2; Cui, Zongbin6; Zhang, Xi1; Meng, Anming3; Sun, Qiang2; Yang, Yufeng1
Corresponding AuthorSun, Qiang(qsun@ion.ac.cn) ; Yang, Yufeng(yangyufeng@outlook.com)
2020-06-04
Source PublicationNUCLEIC ACIDS RESEARCH
ISSN0305-1048
Volume48Issue:10Pages:19
AbstractSite-specific DNA double-strand breaks have been used to generate knock-in through the homology-dependent or -independent pathway. However, low efficiency and accompanying negative impacts such as undesirable indels or tumorigenic potential remain problematic. In this study, we present an enhanced reduced-risk genome editing strategy we named as NEO, which used either site-specific trans or cis double-nicking facilitated by four bacterial recombination factors (RecOFAR). In comparison to currently available approaches, NEO achieved higher knock-in (KI) germline transmission frequency (improving from zero to up to 10% efficiency with an average of 5-fold improvement for 8 loci) and 'cleaner' knock-in of long DNA fragments (up to 5.5 kb) into a variety of genome regions in zebrafish, mice and rats. Furthermore, NEO yielded up to 50% knock-in in monkey embryos and 20% relative integration efficiency in non-dividing primary human peripheral blood lymphocytes (hPBLCs). Remarkably, both on-target and off-target indels were effectively suppressed by NEO. NEO may also be used to introduce low-risk unrestricted point mutations effectively and precisely. Therefore, by balancing efficiency with safety and quality, the NEO method reported here shows substantial potential and improves the in vivo gene-editing strategies that have recently been developed.
DOI10.1093/nar/gkaa195
Funding OrganizationFujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program
Indexed BySCI ; SCI
Language英语
Funding ProjectFujian Province Min-River Scholar Grant[XRC-0947] ; National Natural Science Foundation of China[31071296] ; National Natural Science Foundation of China[31771470] ; National Natural Science Foundation of China[31501083] ; National Natural Science Foundation of China[31970461] ; National Natural Science Foundation of China[31601894] ; National Natural Science Foundation of China[31270854] ; National Natural Science Foundation of China[91432111] ; Fuzhou University Sci-Tech Innovation Grant[XJJ1201] ; Fuzhou University Sci-Tech Innovation Grant[XJ1202] ; Shanghai Municipal Government Bureau of Science and Technology[18JC1410100] ; National Key Research and Development Program of China[2018YFC1003000] ; Startup Funds from Fuzhou University[XRC-1502] ; Fujian Natural Science Foundation[2017J0106] ; Fuzhou University[XRC-1463] ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant[JAT160071] ; National Basic Research Program of China[2011CBA00400] ; CAS Strategic Priority Research Program[XDB02050400]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000569071800003
WOS KeywordHOMOLOGY-DIRECTED REPAIR ; DNA-REPAIR ; KNOCK-IN ; STRANDED-DNA ; CAS9 NICKASE ; BREAK REPAIR ; CRISPR-CAS9 ; ZEBRAFISH ; CRISPR/CAS9 ; PRECISE
PublisherOXFORD UNIV PRESS
Funding OrganizationFujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; Fujian Province Min-River Scholar Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Fuzhou University Sci-Tech Innovation Grant ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; Shanghai Municipal Government Bureau of Science and Technology ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Startup Funds from Fuzhou University ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fujian Natural Science Foundation ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Fuzhou University ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; Education and Scientific Research Projects of Young Teachers in Fujian Province Grant ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program ; CAS Strategic Priority Research Program
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38825
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorSun, Qiang; Yang, Yufeng
Affiliation1.Fuzhou Univ, Inst Life Sci, Fuzhou 350108, Fujian, Peoples R China
2.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Inst Neurosci, State Key Lab Neurosci,CAS Key Lab Primate Neurob, Shanghai 200031, Peoples R China
3.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, State Key Lab Biomembrane & Membrane Engn, Beijing 100084, Peoples R China
4.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China
5.Tsinghua Univ, Zhejiang Prov Key Lab Appl Enzymol, Yangtze Delta Reg Inst, Jiaxing 314006, Zhejiang, Peoples R China
6.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
7.Fujian Med Univ, Union Hosp, Dept Gastr Surg, Fuzhou 350001, Fujian, Peoples R China
Recommended Citation
GB/T 7714
He, Xiaozhen,Chen, Wenfeng,Liu, Zhen,et al. Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species[J]. NUCLEIC ACIDS RESEARCH,2020,48(10):19.
APA He, Xiaozhen.,Chen, Wenfeng.,Liu, Zhen.,Yu, Guirong.,Chen, Youbang.,...&Yang, Yufeng.(2020).Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species.NUCLEIC ACIDS RESEARCH,48(10),19.
MLA He, Xiaozhen,et al."Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species".NUCLEIC ACIDS RESEARCH 48.10(2020):19.
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