IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Identifying difference in primordial germ cells between XX female and XY male yellow catfish embryos
Ge, Si1; Dan, Cheng2; Xiong, Yang1; Gong, Gaorui1; Mei, Jie1; Guo, Wenjie1; Li, Xiaohui1
Corresponding AuthorGuo, Wenjie(guowenjie@mail.hzau.edu.cn) ; Li, Xiaohui(lixiaohui@mail.hzau.edu.cn)
2020-11-30
Source PublicationGENE
ISSN0378-1119
Volume761Pages:7
AbstractPrimordial germ cells (PGCs) are singled out from somatic cells very early during embryogenesis, then they migrate towards the genital ridge and differentiate into gametes through oogenesis or spermatogenesis. Labeling PGCs with Localized RNA expression (LRE) technique by fluorescent proteins has been widely applied among teleost species to study the germ cell development and gonad differentiation. In this study, we first cloned and characterized the 3' untranslated regions (3'UTRs) of nanos homolog 1-like (nos1l), dead end (dnd), and vasa in yellow catfish (Pelteobagrus fulvidraco), and then synthesized the GFP-nosll/dnd/vasa 3'UTR mRNAs. Each of these three 3'UTRs could label PGCs in yellow catfish embryos, of which, vasa 3'UTR exhibited the highest labeling efficiency. To identify the differences in PGCs at embryonic stage, XX all-female and XY all-male yellow catfish embryos were produced and injected with GFP-vasa 3'UTR mRNA. We observed the PGC migration route in these two monosex embryos from 24 hpf to 7 dpf, and found there was no difference between them. Besides, the PGC number was counted at 48 hpf, and the result showed that the average PGC number in XX females (11.3) was significantly larger than that in XY males (8.1). These findings provide an insight into the development of PGCs in yellow catfish embryos and the relationship between embryonic PGC number and the later gonad differentiation.
KeywordPrimordial germ cell Yellow catfish (Pelteobagrus fulvidraco) 3 ' untranslated region Nanos homolog 1-like Dead end Vasa
DOI10.1016/j.gene.2020.145037
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31900585] ; Fundamental Research Funds for the Central Universities[2662018QD051]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000569085200011
WOS KeywordGREEN FLUORESCENT PROTEIN ; SEX-DIFFERENTIATION ; EXPRESSION ; MEDAKA ; GENE ; DIMORPHISM ; VISUALIZATION ; MARKERS
PublisherELSEVIER
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38822
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGuo, Wenjie; Li, Xiaohui
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Ge, Si,Dan, Cheng,Xiong, Yang,et al. Identifying difference in primordial germ cells between XX female and XY male yellow catfish embryos[J]. GENE,2020,761:7.
APA Ge, Si.,Dan, Cheng.,Xiong, Yang.,Gong, Gaorui.,Mei, Jie.,...&Li, Xiaohui.(2020).Identifying difference in primordial germ cells between XX female and XY male yellow catfish embryos.GENE,761,7.
MLA Ge, Si,et al."Identifying difference in primordial germ cells between XX female and XY male yellow catfish embryos".GENE 761(2020):7.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ge, Si]'s Articles
[Dan, Cheng]'s Articles
[Xiong, Yang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ge, Si]'s Articles
[Dan, Cheng]'s Articles
[Xiong, Yang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ge, Si]'s Articles
[Dan, Cheng]'s Articles
[Xiong, Yang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.