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Expression of LamB Vaccine Antigen inWolffia globosa(Duck Weed) Against Fish Vibriosis
Heenatigala, P. P. M.1,2,3; Sun, Zuoliang1,2; Yang, Jingjing1,2; Zhao, Xuyao1,2; Hou, Hongwei1,2
Corresponding AuthorHou, Hongwei(houhw@ihb.ac.cn)
2020-08-20
Source PublicationFRONTIERS IN IMMUNOLOGY
ISSN1664-3224
Volume11Pages:9
AbstractVibriosis is a commonly found bacterial disease identified among fish and shellfish cultured in saline waters. A multitude ofVibriospecies have been identified as the causative agents. LamB, a member of outer membrane protein (OMPs) family of these bacteria is conserved among allVibriospecies and has been identified as an efficient vaccine candidate against vibriosis. Rootless duckweed (Wolffia) is a tiny, edible aquatic plant possessing characteristics suitable for the utilization as a bioreactor. Thus, we attempted to express a protective edible vaccine antigen against fish vibriosis in nuclear-transformedWolffia. We amplifiedLamBgene from virulentVibrio alginolyticusand it was modified to maximize the protein expression level and translocate the protein to the endoplasmic reticulum (ER) in plants. It was cloned into binary vector pMYC under the control of CaMV 35S promoter and introduced intoWolffia globosabyAgrobacterium-mediated transformation. Integration and expression of theLamBgene was confirmed by genomic PCR and RT-PCR. Western blot analysis revealed accumulation of the LamB protein in 8 transgenic lines. The cross-protective property of transgenicWolffiawas evaluated by orally vaccinating zebrafish through feeding fresh transgenicWolffiaand subsequently challenging with virulentV. alginolyticus. High relative percent survival (RPS) of the vaccinated fish (63.3%) confirmed that fish immunized with transgenicWolffiawere well-protected fromVibrioinfection. These findings suggest thatWolffiaexpressed LamB could serve as an edible plant-based candidate vaccine model for fish vibriosis and feasibility of utilizingWolffiaas bioreactor to produce edible vaccines.
Keywordvibriosis Wolffia globosa LamB edible vaccine recombinant protein oral immunization
DOI10.3389/fimmu.2020.01857
Funding OrganizationNational Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; National Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; National Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; National Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Key RD Program[2018YFD0900801] ; Major Project of Technology Innovation Program of Hubei, China[2017ABA135] ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences[AB2018020] ; Hubei Province Postdoctoral Science Foundation
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000567886400001
WOS KeywordOUTER-MEMBRANE PROTEIN ; INFLUENZA-VIRUS H5N1 ; TRANSGENIC DUCKWEED ; PROTECTIVE ANTIGEN ; ORAL IMMUNIZATION ; M2E PEPTIDE ; IMMUNOGENICITY ; IDENTIFICATION ; GLYCOSYLATION ; INDUCTION
PublisherFRONTIERS MEDIA SA
Funding OrganizationNational Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; National Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; National Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; National Key RD Program ; National Key RD Program ; National Key RD Program ; National Key RD Program ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Major Project of Technology Innovation Program of Hubei, China ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Open Project of Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation ; Hubei Province Postdoctoral Science Foundation
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38794
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorHou, Hongwei
Affiliation1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China
3.Natl Aquat Resources Res & Dev Agcy NARA, Inland Aquat Resources & Aquaculture Div IARAD, Colombo, Sri Lanka
Recommended Citation
GB/T 7714
Heenatigala, P. P. M.,Sun, Zuoliang,Yang, Jingjing,et al. Expression of LamB Vaccine Antigen inWolffia globosa(Duck Weed) Against Fish Vibriosis[J]. FRONTIERS IN IMMUNOLOGY,2020,11:9.
APA Heenatigala, P. P. M.,Sun, Zuoliang,Yang, Jingjing,Zhao, Xuyao,&Hou, Hongwei.(2020).Expression of LamB Vaccine Antigen inWolffia globosa(Duck Weed) Against Fish Vibriosis.FRONTIERS IN IMMUNOLOGY,11,9.
MLA Heenatigala, P. P. M.,et al."Expression of LamB Vaccine Antigen inWolffia globosa(Duck Weed) Against Fish Vibriosis".FRONTIERS IN IMMUNOLOGY 11(2020):9.
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