| Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio | |
| Deng, Min1; Dai, Zhili2; Senbati, Yeerken1,3; Li, Lu1; Song, Kang1,3; He, Xugang4 | |
| Corresponding Author | Song, Kang(sk@ihb.ac.cn) |
| 2020-08-04 | |
| Source Publication | FRONTIERS IN MICROBIOLOGY
 |
| ISSN | 1664-302X |
| Volume | 11Pages:11 |
| Abstract | In this study, the effect of aerobic denitrification on nitrogen removal was investigated using two zero-discharge biofloc-based recirculating aquaculture systems with representative carbon-to-nitrogen (C/N) ratios of 15 (CN15) and 20 (CN20). Aquaculture wastewater, residual feed, and fish feces were treated in an aerated suspended growth reactor (SGR, dissolved oxygen > 5.0 mg L-1). Low toxic NH3(<0.1 mg L-1) and NO2--N (<0.5 mg L-1) concentrations and high NO3--N (83.3%) and NO2--N (100%) removal efficiencies were achieved in the fish tank and SGR of CN20, respectively. The nitrogen mass balances indicated that the gaseous nitrogen loss accounted for 72-75% of the nitrogen input. Illumina sequencing and quantitative polymerase chain reaction revealed that increasing the C/N ratio significantly increased the amount of aerobic denitrifying bacteria (Dechloromonas,Rhodobacter,Flavobacterium, andZoogloea) and aerobic denitrifying functional genes (napA,nirK, andnosZ). AutotrophicNitrosomonaswas the dominant nitrifying bacteria in the CN15 system, and autotrophic (Nitrosomonas) and heterotrophic nitrifiers coexisted in the CN20 system. Moreover, the functional prediction analysis showed that the carbohydrate, energy, and amino acid metabolisms in the SGR of the latter increased. In conclusion, aerobic denitrification should widely exist in biofloc systems. |
| Keyword | aerobic denitrification C N ratio nitrogen removal qPCR microbial community function prediction |
| DOI | 10.3389/fmicb.2020.01760 |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[41877344] ; Chinese Academy of Sciences[Y85Z021301] ; Chinese Academy of Sciences[Y951040101] ; Water Pollution Control and Treatment ; National Science and Technology Major Project[2018ZX07208001] ; China Postdoctoral Science Foundation[2019M652738] ; 100 Talents Program of Chinese Academy of Sciences[E029040201] ; 100 Talents Program of Chinese Academy of Sciences[E051040101] |
| WOS Research Area | Microbiology |
| WOS Subject | Microbiology |
| WOS ID | WOS:000563403700001 |
| WOS Keyword | 16S RIBOSOMAL-RNA ; WATER-QUALITY ; SIMULTANEOUS NITRIFICATION ; CARBOHYDRATE ADDITION ; NITRATE REMOVAL ; AMMONIA ; EFFICIENCY ; GENES ; WASTE ; TRANSFORMATIONS |
| Publisher | FRONTIERS MEDIA SA |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/38736 |
| Collection | 水环境工程研究中心_期刊论文 |
| Corresponding Author | Song, Kang |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 2.Anhui Jianzhu Univ, Sch Mat Sci & Chem Engn, Hefei, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China |
| Recommended Citation GB/T 7714 | Deng, Min,Dai, Zhili,Senbati, Yeerken,et al. Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio[J]. FRONTIERS IN MICROBIOLOGY,2020,11:11. |
| APA | Deng, Min,Dai, Zhili,Senbati, Yeerken,Li, Lu,Song, Kang,&He, Xugang.(2020).Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio.FRONTIERS IN MICROBIOLOGY,11,11. |
| MLA | Deng, Min,et al."Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio".FRONTIERS IN MICROBIOLOGY 11(2020):11. |
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