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Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio
Deng, Min1; Dai, Zhili2; Senbati, Yeerken1,3; Li, Lu1; Song, Kang1,3; He, Xugang4
Corresponding AuthorSong, Kang(sk@ihb.ac.cn)
2020-08-04
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume11Pages:11
AbstractIn this study, the effect of aerobic denitrification on nitrogen removal was investigated using two zero-discharge biofloc-based recirculating aquaculture systems with representative carbon-to-nitrogen (C/N) ratios of 15 (CN15) and 20 (CN20). Aquaculture wastewater, residual feed, and fish feces were treated in an aerated suspended growth reactor (SGR, dissolved oxygen > 5.0 mg L-1). Low toxic NH3(<0.1 mg L-1) and NO2--N (<0.5 mg L-1) concentrations and high NO3--N (83.3%) and NO2--N (100%) removal efficiencies were achieved in the fish tank and SGR of CN20, respectively. The nitrogen mass balances indicated that the gaseous nitrogen loss accounted for 72-75% of the nitrogen input. Illumina sequencing and quantitative polymerase chain reaction revealed that increasing the C/N ratio significantly increased the amount of aerobic denitrifying bacteria (Dechloromonas,Rhodobacter,Flavobacterium, andZoogloea) and aerobic denitrifying functional genes (napA,nirK, andnosZ). AutotrophicNitrosomonaswas the dominant nitrifying bacteria in the CN15 system, and autotrophic (Nitrosomonas) and heterotrophic nitrifiers coexisted in the CN20 system. Moreover, the functional prediction analysis showed that the carbohydrate, energy, and amino acid metabolisms in the SGR of the latter increased. In conclusion, aerobic denitrification should widely exist in biofloc systems.
Keywordaerobic denitrification C N ratio nitrogen removal qPCR microbial community function prediction
DOI10.3389/fmicb.2020.01760
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41877344] ; Chinese Academy of Sciences[Y85Z021301] ; Chinese Academy of Sciences[Y951040101] ; Water Pollution Control and Treatment ; National Science and Technology Major Project[2018ZX07208001] ; China Postdoctoral Science Foundation[2019M652738] ; 100 Talents Program of Chinese Academy of Sciences[E029040201] ; 100 Talents Program of Chinese Academy of Sciences[E051040101]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000563403700001
WOS Keyword16S RIBOSOMAL-RNA ; WATER-QUALITY ; SIMULTANEOUS NITRIFICATION ; CARBOHYDRATE ADDITION ; NITRATE REMOVAL ; AMMONIA ; EFFICIENCY ; GENES ; WASTE ; TRANSFORMATIONS
PublisherFRONTIERS MEDIA SA
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; Water Pollution Control and Treatment ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; National Science and Technology Major Project ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences ; 100 Talents Program of Chinese Academy of Sciences
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38736
Collection水环境工程研究中心_期刊论文
Corresponding AuthorSong, Kang
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Anhui Jianzhu Univ, Sch Mat Sci & Chem Engn, Hefei, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Deng, Min,Dai, Zhili,Senbati, Yeerken,et al. Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio[J]. FRONTIERS IN MICROBIOLOGY,2020,11:11.
APA Deng, Min,Dai, Zhili,Senbati, Yeerken,Li, Lu,Song, Kang,&He, Xugang.(2020).Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio.FRONTIERS IN MICROBIOLOGY,11,11.
MLA Deng, Min,et al."Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio".FRONTIERS IN MICROBIOLOGY 11(2020):11.
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