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The spectrin-based membrane skeleton is asymmetric and remodels during neural development in C. elegans
Jia, Ru1,2; Chai, Yongping1,2; Xie, Chao1,2; Liu, Gai3; Zhu, Zhiwen1,2; Huang, Kaiyao3; Li, Wei4; Ou, Guangshuo1,2
Corresponding AuthorLi, Wei(weili_med@mail.tsinghua.edu.cn) ; Ou, Guangshuo(guangshuoou@tsinghua.edu.cn)
2020-08-01
Source PublicationJOURNAL OF CELL SCIENCE
ISSN0021-9533
Volume133Issue:15Pages:10
AbstractPerturbation of spectrin-based membrane mechanics causes hereditary elliptocytosis and spinocerebellar ataxia, but the underlying cellular basis of pathogenesis remains unclear. Here, we introduced conserved disease-associated spectrin mutations into the Caenorhabditis elegans genome and studied the contribution of spectrin to neuronal migration and dendrite formation in developing larvae. The loss of spectrin resulted in ectopic actin polymerization outside of the existing front and secondary membrane protrusions, leading to defective neuronal positioning and dendrite morphology in adult animals. Spectrin accumulated in the lateral region and rear of migrating neuroblasts and redistributes from the soma into the newly formed dendrites, indicating that the spectrin-based membrane skeleton is asymmetric and remodels to regulate actin assembly and cell shape during development. We affinity-purified spectrin from C. elegans and showed that its binding partner ankyrin functions with spectrin. Asymmetry and remodeling of the membrane skeleton might enable spatiotemporal modulation of membrane mechanics for distinct developmental events.
KeywordCell migration Cytoskeleton Dendrite formation Membrane mechanics Spectrin Neural development
DOI10.1242/jcs.248583
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Key R&D Program of China[2017YFA0503501] ; National Key R&D Program of China[2019YFA0508401] ; National Key R&D Program of China[2017YFA0102900] ; National Natural Science Foundation of China[31730052] ; National Natural Science Foundation of China[31525015] ; National Natural Science Foundation of China[31561130153] ; National Natural Science Foundation of China[31671444] ; National Natural Science Foundation of China[31871352]
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000561047900022
WOS KeywordALPHA-SPECTRIN ; CELL-MIGRATION ; ACTIN ; MUTATIONS ; POLARITY
PublisherCOMPANY BIOLOGISTS LTD
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38715
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorLi, Wei; Ou, Guangshuo
Affiliation1.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Beijing Frontier Res Ctr Biol Struct, McGovern Inst Brain Res,Sch Life Sci, Beijing 100084, Peoples R China
2.Tsinghua Univ, MOE Key Lab Prot Sci, Beijing 100084, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
4.Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
Jia, Ru,Chai, Yongping,Xie, Chao,et al. The spectrin-based membrane skeleton is asymmetric and remodels during neural development in C. elegans[J]. JOURNAL OF CELL SCIENCE,2020,133(15):10.
APA Jia, Ru.,Chai, Yongping.,Xie, Chao.,Liu, Gai.,Zhu, Zhiwen.,...&Ou, Guangshuo.(2020).The spectrin-based membrane skeleton is asymmetric and remodels during neural development in C. elegans.JOURNAL OF CELL SCIENCE,133(15),10.
MLA Jia, Ru,et al."The spectrin-based membrane skeleton is asymmetric and remodels during neural development in C. elegans".JOURNAL OF CELL SCIENCE 133.15(2020):10.
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