| High-throughput fluorescence-activated cell sorting for cell wall-deficient microalgal mutants screening | |
| Lin, Juan1,9; Chen, Yi1,8; Yan, Hailong1,8; Nahidian, Bahareh1; Hu, Qiang1,2,3,4,5,6,7; Han, Danxiang1,4,7 | |
| Corresponding Author | Han, Danxiang(danxianghan@ihb.ac.cn) |
| 2020-09-01 | |
| Source Publication | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| ISSN | 2211-9264 |
| Volume | 50Pages:8 |
| Abstract | Bioavailability and extraction efficiency of the natural antioxidant astaxanthin from the unicellular green alga Haematococcus pluvialis have been limited by the rigid algal cell walls. A high-throughput fluorescence-activated cell sorting (FACS) pipeline was developed in this study with the aim to screen cell wall-deficient mutants. The fluorescein-conjugated lectin-Ricinus communis Agglutinin I (RCA(120)) was used as a marker for binding the sugar moieties of the algal cell walls, which enabled discriminating the algal strains that differed in the cell walls. By utilizing the established FACS pipeline, a cell wall-deficient mutant (i.e. mutant-264) with the significantly reduced RCA(120)-fluorescein intensity was screened from the mutant library. The total content of the cell wall-associated sugars was reduced by 43.9% in the mutant-264 as compared to that of the wild type, with significant decrease in mannose, mannitol and galactose. The dry weight, astaxanthin content and extraction efficiency were improved by 21.1%, 13.1% and 16.5%, respectively, in mutant-264 as compared to that of the wild type. This study not only demonstrated the feasibility of utilization of the FACS-based high-throughput pipeline for cell wall mutant screening, but also delivered a novel promising H. pluvialis strain with multiple improved traits for industrial applications. |
| Keyword | Microalgae Cell wall Sugar Lectin High-throughput fluorescence-activated cell sorting Cell wall-deficient mutant |
| DOI | 10.1016/j.algal.2020.102011 |
| Funding Organization | Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Natural Science Foundation of China[31700359] ; Natural Science Foundation of China[31570304] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2017FB18] ; State Development and Investment Corporation of China[Y34115-1-Z01] |
| WOS Research Area | Biotechnology & Applied Microbiology |
| WOS Subject | Biotechnology & Applied Microbiology |
| WOS ID | WOS:000558685000004 |
| WOS Keyword | HAEMATOCOCCUS-PLUVIALIS ; ASTAXANTHIN ; CHLORELLA ; ALGAE ; EXTRACTION |
| Publisher | ELSEVIER |
| Funding Organization | Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China ; State Development and Investment Corporation of China |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/38694 |
| Collection | 藻类生物技术和生物能源研发中心_期刊论文 |
| Corresponding Author | Han, Danxiang |
| Affiliation | 1.Chinese Acad Sci, Ctr Microalgal Biotechnol & Biofuels, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 5.SDIC Biotech Investment Co Ltd, State Dev & Investment Corp, Microalgae Biotechnol Ctr, Beijing 100142, Peoples R China 6.Beijing Key Lab Algae Biomass, SDIC Biotech Investment Corp, Beijing 100142, Peoples R China 7.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100864, Peoples R China 8.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 9.Jiujiang Univ, Poyang Lake Ecoecon Res Ctr, Jiujiang 332005, Peoples R China |
| Recommended Citation GB/T 7714 | Lin, Juan,Chen, Yi,Yan, Hailong,et al. High-throughput fluorescence-activated cell sorting for cell wall-deficient microalgal mutants screening[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2020,50:8. |
| APA | Lin, Juan,Chen, Yi,Yan, Hailong,Nahidian, Bahareh,Hu, Qiang,&Han, Danxiang.(2020).High-throughput fluorescence-activated cell sorting for cell wall-deficient microalgal mutants screening.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,50,8. |
| MLA | Lin, Juan,et al."High-throughput fluorescence-activated cell sorting for cell wall-deficient microalgal mutants screening".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 50(2020):8. |
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