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Production of Highly Active Extracellular Amylase and Cellulase FromBacillus subtilisZIM3 and a Recombinant Strain With a Potential Application in Tobacco Fermentation
Dai, Jingcheng1; Dong, Aijun2; Xiong, Guoxi2; Liu, Yaqi1,3; Hossain, Md Shahdat1,4,5; Liu, Shuangyuan1,4; Gao, Na1,4; Li, Shuyang1,4; Wang, Jing1,4; Qiu, Dongru1,4
Corresponding AuthorDong, Aijun(11013213@hbtobacco.cn) ; Qiu, Dongru(qiu@ihb.ac.cn)
2020-07-21
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume11Pages:14
AbstractIn this study, a series of bacteria capable of degrading starch and cellulose were isolated from the aging flue-cured tobacco leaves. Remarkably, there was a thermophilic bacterium,Bacillus subtilisZIM3, that can simultaneously degrade both starch and cellulose at a wide range of temperature and pH values. Genome sequencing, comparative genomics analyses, and enzymatic activity assays showed that the ZIM3 strain expressed a variety of highly active plant biomass-degrading enzymes, such as the amylase AmyE1 and cellulase CelE1. Thein vitroand PhoA-fusion assays indicated that these enzymes degrading complex plant biomass into fermentable sugars were secreted into ambient environment to function. Besides, the amylase and cellulase activities were further increased by three- to five-folds by using overexpression. Furthermore, a fermentation strategy was developed and the biodegradation efficiency of the starch and cellulose in the tobacco leaves were improved by 30-48%. These results reveal thatB. subtilisZIM3 and the recombinant strain exhibited high amylase and cellulase activities for efficient biodegradation of starch and cellulose in tobacco and could potentially be applied for industrial tobacco fermentation.
Keywordamylase cellulase heterogeneous expression bacillus tobacco
DOI10.3389/fmicb.2020.01539
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[91751106] ; Key Research Program of the Chinese Academy of Sciences[XDA23040401] ; Key Research Program of the Chinese Academy of Sciences[Y15103-1-401] ; Enterprise Principal Project[Y84108]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000558895600001
WOS KeywordTHERMOSTABLE ALPHA-AMYLASE ; BACILLUS ; INOCULATION ; REAGENT ; LEAVES ; GENES ; WASTE
PublisherFRONTIERS MEDIA SA
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project ; Enterprise Principal Project
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38688
Collection其他_期刊论文
Corresponding AuthorDong, Aijun; Qiu, Dongru
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
2.China Tobacco Hubei Ind Co Ltd, Technol Res Ctr, Wuhan, Peoples R China
3.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Natl Inst Biotechnol, Dhaka, Bangladesh
Recommended Citation
GB/T 7714
Dai, Jingcheng,Dong, Aijun,Xiong, Guoxi,et al. Production of Highly Active Extracellular Amylase and Cellulase FromBacillus subtilisZIM3 and a Recombinant Strain With a Potential Application in Tobacco Fermentation[J]. FRONTIERS IN MICROBIOLOGY,2020,11:14.
APA Dai, Jingcheng.,Dong, Aijun.,Xiong, Guoxi.,Liu, Yaqi.,Hossain, Md Shahdat.,...&Qiu, Dongru.(2020).Production of Highly Active Extracellular Amylase and Cellulase FromBacillus subtilisZIM3 and a Recombinant Strain With a Potential Application in Tobacco Fermentation.FRONTIERS IN MICROBIOLOGY,11,14.
MLA Dai, Jingcheng,et al."Production of Highly Active Extracellular Amylase and Cellulase FromBacillus subtilisZIM3 and a Recombinant Strain With a Potential Application in Tobacco Fermentation".FRONTIERS IN MICROBIOLOGY 11(2020):14.
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