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NLRC3-like 1 inhibits NOD1-RIPK2 pathway via targeting RIPK2
Fang, Hong1,3; Wu, Xiao Man1,3; Hu, Yi Wei1,3; Song, Yun Jie1,3; Zhang, Jie1; Chang, Ming Xian1,2,3
Corresponding AuthorChang, Ming Xian(mingxianchang@ihb.ac.cn)
2020-11-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume112Pages:12
AbstractBoth NLRC3 and NOD1 belong to regulatory NLR subfamily based on their best-characterized function. In mammals, NLRC3 was reported to function by attenuating signaling cascades initiated by other families of PRRs. In teleosts, multiple NLRC3-like genes were identified through transcriptome sequencing. However, the functions of many NLRC3-like genes, especially the fish-specific NLRC3-like genes, remain unclear. In the present study, we report the functional characterization of a novel category of NLRC3-like proteins (named as NLRC3-like 1) from the zebrafish, which consists of a fish-specific FISNA, a conserved NACHT and five C-terminal LRRs domains. The expression of zebrafish NLRC3-like 1 was inducible in response to Edwardsiella piscicida infection. During bacterial infection, the in vitro and in vivo studies revealed that zebrafish NLRC3-like 1 overexpression facilitated bacterial growth and dissemination, together with the decreased survival rate of zebrafish larvae infected with E. piscicida. The attenuated response by zebrafish NLRC3-like 1 in response to bacterial infection were characterized by the impaired expression of antibacterial genes, proinflammatory cytokines and Nox genes. Furthermore, zebrafish NLRC3-like 1 interacted with the adaptor protein RIPK2 of NODs signaling via the FISNA (Fish-specific NACHT associated domain) and NACHT domains. However, the interaction between zebrafish NLRC3-like 1 and RIPK2 inhibited the assembly of the NOD1-RIPK2 complex. Importantly, zebrafish NLRC3-like 1 inhibited NOD1-mediated antibacterial activity, NF-kappa B and MAPK pathways and proinflammatory cytokine production. All together, these results firstly demonstrate that zebrafish NLRC3-like 1 inhibits NOD1-RIPK2 antibacterial pathway via targeting the adaptor protein RIPK2.
KeywordZebrafish NLRC3-like Proinflammatory cytokines NOD1-RIPK2 pathway Negative regulation
DOI10.1016/j.dci.2020.103769
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31672687] ; National Natural Science Foundation of China[31873046] ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology[2019FBZ04]
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000564711100004
WOS KeywordNOD-LIKE RECEPTOR ; NF-KAPPA-B ; MOLECULAR CHARACTERIZATION ; AEROMONAS-HYDROPHILA ; FUNCTIONAL-ANALYSIS ; EXPRESSION ANALYSIS ; IMMUNE-RESPONSE ; MIIUY CROAKER ; FAMILY-MEMBER ; NLR FAMILY
PublisherELSEVIER SCI LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund for State Key Laboratory of Freshwater Ecology and Biotechnology
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38629
Collection其他_期刊论文
Corresponding AuthorChang, Ming Xian
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Fang, Hong,Wu, Xiao Man,Hu, Yi Wei,et al. NLRC3-like 1 inhibits NOD1-RIPK2 pathway via targeting RIPK2[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,112:12.
APA Fang, Hong,Wu, Xiao Man,Hu, Yi Wei,Song, Yun Jie,Zhang, Jie,&Chang, Ming Xian.(2020).NLRC3-like 1 inhibits NOD1-RIPK2 pathway via targeting RIPK2.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,112,12.
MLA Fang, Hong,et al."NLRC3-like 1 inhibits NOD1-RIPK2 pathway via targeting RIPK2".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 112(2020):12.
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