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Assessing the intestinal bacterial community of farmed Nile tilapia (Oreochromis niloticus) by high-throughput absolute abundance quantification
Wu, Zhenbing1,2; Wang, Shuyi1,2; Zhang, Qianqian1,3; Hao, Jingwen1,2; Lin, Yaoyao1,2; Zhang, Jinyong1,3; Li, Aihua1,3
Corresponding AuthorLi, Aihua(liaihua@ihb.ac.cn)
2020-12-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume529Pages:9
AbstractIntestinal microbiota studies based on high-throughput sequencing have attracted increasing attention due to its vital impact on host health. However, the taxonomical profile in relative proportion does not reflect the quantity of the intestinal bacteria and the inter-sample differences among taxa. In this study, we applied a high-throughput absolute abundance quantification method that used an internal standard strain to simultaneously obtain both the relative and absolute abundances in the intestinal bacterial community of farmed Nile tilapia (Oreochromis niloticus). The results showed that the structure, relative and absolute abundances, and function of the intestinal indigenous microbiota didn't change significantly after adding a low concentration of internal standard strain, and a comprehensive quantitative intestinal bacterial ecology was obtained. We first found that the total absolute abundance of the intestinal indigenous bacteria in farmed Nile tilapia was 2.95 x 10(8) cells g(-1), and most genera concentrations were in the range of 10(3) to 10(5) cells g(-1). The intestinal bacterial community was dominated by Cetobacterium with a concentration of 10(8) cells g(-1), followed by Plesiomonas, Romboutsia, and Escherichia-Shigella with concentrations of over 10(7) cells g(-1). The potential intestinal pathogens Aeromonas and Streptococcus reached 10(5) cells g(-1) in the tilapia intestines, while the potential probiotics such as Lactobacillus and Enterococcus with a high concentration of over 10(6) cells g(-1) were detected. Furthermore, PICRUSt analysis revealed that functional genes involved in membrane transport, carbohydrate metabolism, and amino acid metabolism were enriched in the intestinal microbiota of tilapia. This study characterized the intestinal quantitative bacterial ecology of farmed Nile tilapia, providing foundational support for an in-depth understanding of the intestinal microbiota and its interaction mechanism with the host.
KeywordIntestinal bacterial community Nile tilapia (Oreochromis niloticus) Internal standard strain Absolute abundance Quantitative bacterial ecology
DOI10.1016/j.aquaculture.2020.735688
Funding OrganizationKey Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project
Indexed BySCI ; SCI
Language英语
Funding ProjectKey Project of Scientific & Technological Innovation of Hubei Province[2018ABA101] ; Wuhan Science and Technology Project[2019020701011480] ; FEBL project[2016FBZ04] ; Jiangsu Marine and Fisheries Bureau project[D2015-11] ; Jiangsu Marine and Fisheries Bureau project[D2017-3]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000566469700016
WOS KeywordMICROBIAL COMMUNITIES ; GUT MICROBIOME ; WATER ; METABOLISM ; STANDARD ; REVEALS ; FISH ; CARP ; PCR
PublisherELSEVIER
Funding OrganizationKey Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Key Project of Scientific & Technological Innovation of Hubei Province ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; Wuhan Science and Technology Project ; FEBL project ; FEBL project ; FEBL project ; FEBL project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project ; Jiangsu Marine and Fisheries Bureau project
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38623
Collection水环境工程研究中心_期刊论文
Corresponding AuthorLi, Aihua
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Minist Agr, Key Lab Aquaculture Dis Control, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wu, Zhenbing,Wang, Shuyi,Zhang, Qianqian,et al. Assessing the intestinal bacterial community of farmed Nile tilapia (Oreochromis niloticus) by high-throughput absolute abundance quantification[J]. AQUACULTURE,2020,529:9.
APA Wu, Zhenbing.,Wang, Shuyi.,Zhang, Qianqian.,Hao, Jingwen.,Lin, Yaoyao.,...&Li, Aihua.(2020).Assessing the intestinal bacterial community of farmed Nile tilapia (Oreochromis niloticus) by high-throughput absolute abundance quantification.AQUACULTURE,529,9.
MLA Wu, Zhenbing,et al."Assessing the intestinal bacterial community of farmed Nile tilapia (Oreochromis niloticus) by high-throughput absolute abundance quantification".AQUACULTURE 529(2020):9.
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