| The interplay between chromophore and protein determines the extended excited state dynamics in a single-domain phytochrome | |
| Slavov, Chavdar1; Fischer, Tobias1; Barnoy, Avishai2; Shin, Heewhan3; Rao, Aditya G.2; Wiebeler, Christian2,5,7; Zeng, Xiaoli3,8; Sun, Yafang4; Xu, Qianzhao5; Gutt, Alexander6; Zhao, Kai-Hong4; Gaertner, Wolfgang5,6; Yang, Xiaojing3; Schapiro, Igor2; Wachtveitl, Josef1 | |
| Corresponding Author | Slavov, Chavdar(chslavov@theochem.uni-frankfurt.de) ; Yang, Xiaojing(xiaojing@uic.edu) ; Schapiro, Igor(igor.schapiro@mail.huji.ac.il) ; Wachtveitl, Josef(wveitl@theochem.uni-frankfurt.de) |
| 2020-07-14 | |
| Source Publication | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| ISSN | 0027-8424 |
| Volume | 117Issue:28Pages:16356-16362 |
| Abstract | Phytochromes are a diverse family of bilin-binding photoreceptors that regulate a wide range of physiological processes. Their pho-tochemical properties make them attractive for applications in optogenetics and superresolution microscopy. Phytochromes un-dergo reversible photoconversion triggered by the Z reversible arrow E photo-isomerization about the double bond in the bilin chromophore. However, it is not fully understood at the molecular level how the protein framework facilitates the complex photoisomerization dynamics. We have studied a single-domain bilin-binding photo-receptor All2699g1 (Nostoc sp. PCC 7120) that exhibits photocon-version between the red light-absorbing (Pr) and far red-absorbing (Pfr) states just like canonical phytochromes. We present the crys-tal structure and examine the photoisomerization mechanism of the Pr form as well as the formation of the primary photoproduct Lumi-R using time-resolved spectroscopy and hybrid quantum me-chanics/molecular mechanics simulations. We show that the un-usually long excited state lifetime (broad lifetime distribution centered at similar to 300 picoseconds) is due to the interactions between the isomerizing pyrrole ring D and an adjacent conserved Tyr142. The decay kinetics shows a strongly distributed character which is imposed by the nonexponential protein dynamics. Our findings offer a mechanistic insight into how the quantum efficiency of the bilin photoisomerization is tuned by the protein environ-ment, thereby providing a structural framework for engineer-ing bil in-based optical agents for imaging and optogenetics applications. |
| Keyword | knotless phytochrome photoisomerization ultrafast spectroscopy X-ray structure QM/MM |
| DOI | 10.1073/pnas.192170611 |
| Funding Organization | Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Deutsche Forschungsgemeinschaft[WI 4853/1-1] ; Deutsche Forschungsgemeinschaft[WI 4853/2-1] ; Deutsche Forschungsgemeinschaft[WA 1850/4-2] ; National Natural Science Foundation of China (NSFC)[31861143029] ; National Natural Science Foundation of China (NSFC)[44131770822] ; European Research Council (Horizon 2020)[678169] ; Protonation Dynamics in Protein Function (Mercator fellowship)[SFB 1078] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000553289400011 |
| WOS Keyword | GAF DOMAIN ; FEMTOSECOND ; BACTERIOPHYTOCHROME ; PHOTODYNAMICS ; CYANOBACTERIOCHROMES ; PHYCOCYANOBILIN ; PHOTOCONVERSION ; PHOTOCHEMISTRY ; HETEROGENEITY ; DIVERSE |
| Publisher | NATL ACAD SCIENCES |
| Funding Organization | Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; European Research Council (Horizon 2020) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) ; Protonation Dynamics in Protein Function (Mercator fellowship) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/38604 |
| Collection | 藻类生物学及应用研究中心_期刊论文 |
| Corresponding Author | Slavov, Chavdar; Yang, Xiaojing; Schapiro, Igor; Wachtveitl, Josef |
| Affiliation | 1.Goethe Univ, Inst Phys & Theoret Chem, D-60438 Frankfurt, Germany 2.Hebrew Univ Jerusalem, Inst Chem, Fritz Haber Ctr Mol Dynam Res, IL-9190401 Jerusalem, Israel 3.Univ Illinois, Dept Chem, Chicago, IL 60607 USA 4.Huazhong Agr Univ, Key State Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China 5.Univ Leipzig, Inst Analyt Chem, D-04103 Leipzig, Germany 6.Max Planck Inst Chem Energy Convers, D-45470 Mulheim, Germany 7.Leibniz Inst Surface Engn, D-04318 Leipzig, Germany 8.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China |
| Recommended Citation GB/T 7714 | Slavov, Chavdar,Fischer, Tobias,Barnoy, Avishai,et al. The interplay between chromophore and protein determines the extended excited state dynamics in a single-domain phytochrome[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2020,117(28):16356-16362. |
| APA | Slavov, Chavdar.,Fischer, Tobias.,Barnoy, Avishai.,Shin, Heewhan.,Rao, Aditya G..,...&Wachtveitl, Josef.(2020).The interplay between chromophore and protein determines the extended excited state dynamics in a single-domain phytochrome.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,117(28),16356-16362. |
| MLA | Slavov, Chavdar,et al."The interplay between chromophore and protein determines the extended excited state dynamics in a single-domain phytochrome".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117.28(2020):16356-16362. |
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