| A rapid method of sex-specific marker discovery based on NGS and determination of the XX / XY sex-determination system in Channa maculata | |
| Yang, Cheng1,2; Huang, Rong1; Ou, Mi3; Gui, Bin1; Zhao, Jian3; He, Libo1; Li, Yongming1; Liao, Lanjie1; Chen, Kunci3; Wang, Yaping1,4 | |
| Corresponding Author | Huang, Rong(huangrong@ihb.ac.cn) ; Wang, Yaping(wangyp.ihb@gmail.com) |
| 2020-11-15 | |
| Source Publication | AQUACULTURE
 |
| ISSN | 0044-8486 |
| Volume | 528Pages:6 |
| Abstract | The discovery of sex-specific markers is of great significance for the study of unisexual breeding and sex-determination mechanisms of fish. The all-male hybrid snakehead is a freshwater aquaculture species with high economic value. The breeding of all-male hybrid snakeheads depends on the discovery of sex-specific markers of Taiwan snakeheads. In this study, 24 females and 24 males of Taiwan snakeheads were sequenced by NGS, and then a set of biological information analysis process was developed to speculate that Taiwan snakeheads were XX / XY sex-determining system, and 1250 male-related contigs were assembled; In combination with indels and SNP sites analysis, Chelex 100 DNA extraction and an HRM scan, two molecular markers (CM286 and CM1053) were selected from 29 male-related contigs, which could clearly distinguish XX females, XY males, and YY supermales; Then, it was confirmed by Sanger sequencing that Taiwan snakeheads had a male heterogamete type, and the ratio of females to males in three full-sib families was all close to 1:1 (P >.05). Therefore, Taiwan snakeheads were determined to be the XX / XY sex-determining system. The results showed that the effective sex-specific markers, combined with the Chelex 100 DNA extraction and HRM scan technology, could realize high-throughput and rapid detection of the three sexes (XX, XY, and YY) of Taiwan snakeheads, and could be directly applied to the breeding of the all-male hybrid snakehead. This process of bioinformatic analysis for sexspecific marker discovery is simple and efficient, and can also be applied to the study of other fish sex-specific marker discovery and sex-determination mechanisms. |
| Keyword | Sex-specific marker Next-generation sequencing (NGS) Sex-determination mechanism Unisexual breeding |
| DOI | 10.1016/j.aquaculture.2020.735499 |
| Funding Organization | National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Key Research & Development Program of China[2018YFD0901201] ; National Natural Science Foundation of China[31672647] |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000553693000007 |
| WOS Keyword | PROVIDES INSIGHTS ; IDENTIFICATION ; GENOME ; DIFFERENTIATION ; DIVERSITY ; EVOLUTION ; CATFISH ; FISH |
| Publisher | ELSEVIER |
| Funding Organization | National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Key Research & Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/38586 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Huang, Rong; Wang, Yaping |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr, Guangzhou 510380, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China |
| Recommended Citation GB/T 7714 | Yang, Cheng,Huang, Rong,Ou, Mi,et al. A rapid method of sex-specific marker discovery based on NGS and determination of the XX / XY sex-determination system in Channa maculata[J]. AQUACULTURE,2020,528:6. |
| APA | Yang, Cheng.,Huang, Rong.,Ou, Mi.,Gui, Bin.,Zhao, Jian.,...&Wang, Yaping.(2020).A rapid method of sex-specific marker discovery based on NGS and determination of the XX / XY sex-determination system in Channa maculata.AQUACULTURE,528,6. |
| MLA | Yang, Cheng,et al."A rapid method of sex-specific marker discovery based on NGS and determination of the XX / XY sex-determination system in Channa maculata".AQUACULTURE 528(2020):6. |
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