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Functional Dissection of Genes Encoding DNA Polymerases Based on Conditional Mutants in the Heterocyst-Forming CyanobacteriumAnabaenaPCC 7120
Xing, Wei-Yue1,2,3; Xie, Li-rui1,2; Zeng, Xiaoli1,2; Yang, Yiling1,2; Zhang, Cheng-Cai1,2,4,5
Corresponding AuthorYang, Yiling(yangyiling@ihb.ac.cn) ; Zhang, Cheng-Cai(cczhang@ihb.ac.cn)
2020-06-03
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume11Pages:13
AbstractThe filamentous cyanobacteriumAnabaenasp. PCC 7120 develops N-2-fixing heterocyst cells under condition of combined-nitrogen deprivation and constitutes an excellent model for studying cell differentiation. The mechanism of heterocyst development has been extensively investigated and a network of regulating factors has been identified. A few studies have showed that the process of heterocyst differentiation relates with cell cycle events, but further investigation is still required to understand this relationship. In a previous study, we created a conditional mutant of PolI encoding gene,polA, by using a CRISPR/Cpf1 gene-editing technique. Here, we were able to create another conditional mutant of a PolIII encoding genednaENIusing a similar strategy and subsequently confirmed the essential roles of bothpolAanddnaENIin DNA replication. Further investigation on the phenotype of the mutants showed that lack of PolI caused defects in chromosome segregation and cell division, while lack of DnaENI (PolIII) prevented bulk DNA synthesis, causing significant loss of DNA content. Our findings also suggested the possible existence of a SOS-response like mechanism operating inAnabaenaPCC 7120. Moreover, we found that heterocyst development was differently affected in the two conditional mutants, with double heterocysts/proheterocysts found in PolI conditional mutant. We further showed that formation of such double heterocysts/proheterocysts are likely caused by the difficulty in nucleoids segregation, resulting delayed, or non-complete closure of the septum between the two daughter cells. This study uncovers a link between DNA replication process and heterocyst differentiation, paving the way for further studies on the relationship between cell cycle and cell development.
Keywordheterocyst DNA replication cell cycle SOS response cyanobacteria
DOI10.3389/fmicb.2020.01108
Funding OrganizationKey Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences
Indexed BySCI ; SCI
Language英语
Funding ProjectKey Research Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDJ-SSW-SMC016] ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000543342600001
WOS KeywordNITROGEN-FIXING CYANOBACTERIUM ; PATTERN-FORMATION ; ESCHERICHIA-COLI ; ANABAENA ; DIFFERENTIATION ; LEXA ; RECA ; MUTAGENESIS ; EXPRESSION ; PROTEINS
PublisherFRONTIERS MEDIA SA
Funding OrganizationKey Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences ; Program of Innovative Academy for Seed Design, Chinese Academy of Sciences
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38382
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorYang, Yiling; Zhang, Cheng-Cai
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China
2.Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Aix Marseille Univ, Inst WUT AMU, Marseille, France
5.Wuhan Univ Technol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Xing, Wei-Yue,Xie, Li-rui,Zeng, Xiaoli,et al. Functional Dissection of Genes Encoding DNA Polymerases Based on Conditional Mutants in the Heterocyst-Forming CyanobacteriumAnabaenaPCC 7120[J]. FRONTIERS IN MICROBIOLOGY,2020,11:13.
APA Xing, Wei-Yue,Xie, Li-rui,Zeng, Xiaoli,Yang, Yiling,&Zhang, Cheng-Cai.(2020).Functional Dissection of Genes Encoding DNA Polymerases Based on Conditional Mutants in the Heterocyst-Forming CyanobacteriumAnabaenaPCC 7120.FRONTIERS IN MICROBIOLOGY,11,13.
MLA Xing, Wei-Yue,et al."Functional Dissection of Genes Encoding DNA Polymerases Based on Conditional Mutants in the Heterocyst-Forming CyanobacteriumAnabaenaPCC 7120".FRONTIERS IN MICROBIOLOGY 11(2020):13.
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