IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803
Wu, Dongqing1,2,3; Wang, Yali1,2; Xu, Xudong1,2
Corresponding AuthorXu, Xudong(xux@ihb.ac.cn)
2020-06-09
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume11Pages:8
AbstractIncreasing evidence has shown that DNA methylation is involved in gene regulation in prokaryotes. However, there have been very limited reports about the role of DNA methylation in regulation of gene expression and physiological functions in cyanobacteria. InSynechocystissp. PCC 6803, four genes on the plasmid pSYSX are predicted to encode the type I restriction-methylation system,slr6095andslr6096for the M subunit,slr6097for the S subunit andslr6102for theRsubunit. Compared to the wild type,slr6095,slr6096, andslr6097mutants lacked the GG(m6)AN(7)TTGG/CCA(m6)AN(7)TCC methylation in genomic DNA. Transcriptomic analysis indicated that 171 genes were reproducibly up- or down-regulated in all three mutants relative to the wild type. The changed expression of some genes, includingsll1356for glycogen phosphorylase (GlgP), was associated with the loss of GG(m6)AN(7)TTGG/CCA(m6)AN(7)TCC methylation in the coding regions or the upstream non-coding sequences. Inactivation ofslr6095,slr6096, orslr6097increased the expression ofsll1356and the GlgP activity but lowered the glycogen content. These results indicated that the DNA methylation by a type I RM system could alter the expression of certain genes and physiological functions inSynechocystissp. PCC 6803.
KeywordSynechocystis type I RM system gene regulation glgP glycogen catabolism
DOI10.3389/fmicb.2020.01258
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31470203] ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS[2019FBZ09] ; Knowledge Innovation Project of Hubei Province[2017CFA021]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000543513600001
WOS KeywordDNA METHYLTRANSFERASES ; BACTERIAL GLYCOGEN ; SYNECHOCYSTIS ; TOLERANCE ; STRESS
PublisherFRONTIERS MEDIA SA
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/38373
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, Xudong
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wu, Dongqing,Wang, Yali,Xu, Xudong. Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803[J]. FRONTIERS IN MICROBIOLOGY,2020,11:8.
APA Wu, Dongqing,Wang, Yali,&Xu, Xudong.(2020).Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803.FRONTIERS IN MICROBIOLOGY,11,8.
MLA Wu, Dongqing,et al."Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803".FRONTIERS IN MICROBIOLOGY 11(2020):8.
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