| Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803 | |
| Wu, Dongqing1,2,3; Wang, Yali1,2; Xu, Xudong1,2 | |
| Corresponding Author | Xu, Xudong(xux@ihb.ac.cn) |
| 2020-06-09 | |
| Source Publication | FRONTIERS IN MICROBIOLOGY
 |
| ISSN | 1664-302X |
| Volume | 11Pages:8 |
| Abstract | Increasing evidence has shown that DNA methylation is involved in gene regulation in prokaryotes. However, there have been very limited reports about the role of DNA methylation in regulation of gene expression and physiological functions in cyanobacteria. InSynechocystissp. PCC 6803, four genes on the plasmid pSYSX are predicted to encode the type I restriction-methylation system,slr6095andslr6096for the M subunit,slr6097for the S subunit andslr6102for theRsubunit. Compared to the wild type,slr6095,slr6096, andslr6097mutants lacked the GG(m6)AN(7)TTGG/CCA(m6)AN(7)TCC methylation in genomic DNA. Transcriptomic analysis indicated that 171 genes were reproducibly up- or down-regulated in all three mutants relative to the wild type. The changed expression of some genes, includingsll1356for glycogen phosphorylase (GlgP), was associated with the loss of GG(m6)AN(7)TTGG/CCA(m6)AN(7)TCC methylation in the coding regions or the upstream non-coding sequences. Inactivation ofslr6095,slr6096, orslr6097increased the expression ofsll1356and the GlgP activity but lowered the glycogen content. These results indicated that the DNA methylation by a type I RM system could alter the expression of certain genes and physiological functions inSynechocystissp. PCC 6803. |
| Keyword | Synechocystis type I RM system gene regulation glgP glycogen catabolism |
| DOI | 10.3389/fmicb.2020.01258 |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31470203] ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS[2019FBZ09] ; Knowledge Innovation Project of Hubei Province[2017CFA021] |
| WOS Research Area | Microbiology |
| WOS Subject | Microbiology |
| WOS ID | WOS:000543513600001 |
| WOS Keyword | DNA METHYLTRANSFERASES ; BACTERIAL GLYCOGEN ; SYNECHOCYSTIS ; TOLERANCE ; STRESS |
| Publisher | FRONTIERS MEDIA SA |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/38373 |
| Collection | 藻类生物学及应用研究中心_期刊论文 |
| Corresponding Author | Xu, Xudong |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Wu, Dongqing,Wang, Yali,Xu, Xudong. Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803[J]. FRONTIERS IN MICROBIOLOGY,2020,11:8. |
| APA | Wu, Dongqing,Wang, Yali,&Xu, Xudong.(2020).Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803.FRONTIERS IN MICROBIOLOGY,11,8. |
| MLA | Wu, Dongqing,et al."Effects of a Type I RM System on Gene Expression and Glycogen Catabolism inSynechocystissp. PCC 6803".FRONTIERS IN MICROBIOLOGY 11(2020):8. |
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