IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY
Zhang Yongan1; Nie Pin1
2002
Source Publication中国海洋湖沼学报:英文版
ISSN0254-4059
Volume020Issue:004Pages:332
AbstractSerum immunoglobulin from the mandarin fish,or the so-called Chinese perch,Siniperca chuatsi(Basilewsky),was successfully purified using affinity chromatography.Heavy and light chains were detected on electrophoresis gel,with molecular weights being estimated at 72 and 29 kDa,respectively.The tetrameric IgM of S.chuatsi was calculated to be 808 kDa.The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Westem blot analysis.The antisera reacted strongly with the heavy chains of S.chuatsi immunoglobulin.Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody.
Keyword免疫球蛋白 中国金鲈 多克隆抗体 体液免疫反应 鱼类
Language英语
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/37836
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation1.Institute of Hydrobiology, Chinese Academy of Sciences
2.Institute of Hydrobiology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang Yongan,Nie Pin. SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY[J]. 中国海洋湖沼学报:英文版,2002,020(004):332.
APA Zhang Yongan,&Nie Pin.(2002).SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY.中国海洋湖沼学报:英文版,020(004),332.
MLA Zhang Yongan,et al."SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY".中国海洋湖沼学报:英文版 020.004(2002):332.
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