| SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY | |
| Zhang Yongan1; Nie Pin1 | |
| 2002 | |
| Source Publication | 中国海洋湖沼学报：英文版
 |
| ISSN | 0254-4059 |
| Volume | 020Issue:004Pages:332 |
| Abstract | Serum immunoglobulin from the mandarin fish,or the so-called Chinese perch,Siniperca chuatsi(Basilewsky),was successfully purified using affinity chromatography.Heavy and light chains were detected on electrophoresis gel,with molecular weights being estimated at 72 and 29 kDa,respectively.The tetrameric IgM of S.chuatsi was calculated to be 808 kDa.The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Westem blot analysis.The antisera reacted strongly with the heavy chains of S.chuatsi immunoglobulin.Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody. |
| Keyword | 免疫球蛋白 中国金鲈 多克隆抗体 体液免疫反应 鱼类 |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/37836 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Affiliation | 1.Institute of Hydrobiology, Chinese Academy of Sciences 2.Institute of Hydrobiology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zhang Yongan,Nie Pin. SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY[J]. 中国海洋湖沼学报：英文版,2002,020(004):332. |
| APA | Zhang Yongan,&Nie Pin.(2002).SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY.中国海洋湖沼学报：英文版,020(004),332. |
| MLA | Zhang Yongan,et al."SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY".中国海洋湖沼学报：英文版 020.004(2002):332. |
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