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鲤鱼3个亚种线粒体DNA ND5/6区段的PCR—RFLP分析及亚种间分子标记的确立
周建峰; 王忠卫; 吴清江
2003
Source Publication科学通报
ISSN0023-074X
Volume048Issue:002Pages:167
Abstract从鲤鱼3个亚种(Cyprinus carpio carpio,Cyprinus carpio haematopterus和Cyprinus carpio rubrofuscus)中选出具代表性的品系共10个,运用PCR方法扩增出2.4kb的mtDNA ND5/6区段,共用9种限制性内切酶进行酶切分析。结果表明,每个亚种拥有一种单倍型,有4种限制性内切酶(DdeⅠ,HaeⅢ,TaqⅠ和MboⅠ)酶切后产生了能将3个亚种区分开来的诊断性限制性酶切位点,可利用其作为鉴定3个亚种的遗传标记和遗传育种的辅助手段。
Keyword鲤鱼 亚种 线粒体DNA 限制性内切酶 ND5/6区段 PCR-RFLP分析 分子遗传标记 淡水养殖
Language英语
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/37159
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation中国科学院水生生物研究所
Recommended Citation
GB/T 7714
周建峰,王忠卫,吴清江. 鲤鱼3个亚种线粒体DNA ND5/6区段的PCR—RFLP分析及亚种间分子标记的确立[J]. 科学通报,2003,048(002):167.
APA 周建峰,王忠卫,&吴清江.(2003).鲤鱼3个亚种线粒体DNA ND5/6区段的PCR—RFLP分析及亚种间分子标记的确立.科学通报,048(002),167.
MLA 周建峰,et al."鲤鱼3个亚种线粒体DNA ND5/6区段的PCR—RFLP分析及亚种间分子标记的确立".科学通报 048.002(2003):167.
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