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Depletion of insulin receptors leads to b-cell hyperplasia in zebrafish
Yang Binyuan1; Zhai Gang1; Gong Yulong1; Su Jingzhi1; Han Dong1; Yin Zhan1; Xie Shouqi1
2017
Source PublicationScience Bulletin
ISSN2095-9273
Volume62Issue:7Pages:486
AbstractHyperglycemia in type 2 diabetes results from an inability of insulin to regulate gluconeogenesis. To characterize the role of the insulin/insulin receptor pathway in glycometabolism and type 2 diabetes, we created a zebrafish model in which insulin receptors a and b (insra and insrb) have been ablated. We first observed that insra and insrb were both expressed abundantly during embryonic development and in various adult tissues. Increased expression of insulin and number of β-cells were observed in insra-/-/ insrb-/- fish together with higher glucose in insra-/-, insrb-/-, or insra-/-/insrb-/- fish, indicating that insra and insrb were knocked out effectively. However, compared to the wild-type fish, insra-/-/ insrb-/- fish died between 5 and 16 days post-fertilization (dpf) with severe pericardial edema and increased level of cell apoptosis, which was not induced by increased total body glucose content. Increased gluconeogenesis and decreased glycolysis were also observed in both single and double knockout fish, but no mortality or malformation was observed in single knockout fish. Given the importance of insulin receptors in glucose homeostasis and embryonic development, transcriptome analysis was used to provide an important model of defective insulin signaling and to study its developmental consequences in zebrafish. The results indicated that both insra and insrb played a pivotal role in glucose metabolism and embryonic development, and insra was more critical than insrb in the insulin signaling pathway.
KeywordHyperglycemia Type 2 diabetes Zebrafish b-cells Insulin receptors
Language英语
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/37010
Collection淡水生态学研究中心_期刊论文
Affiliation1.Institute of Hydrobiology, Chinese Academy of Sciences
2.Institute of Hydrobiology, Chinese Academy of Sciences
3.Institute of Hydrobiology, Chinese Academy of Sciences
4.Institute of Hydrobiology, Chinese Academy of Sciences
5.Institute of Hydrobiology, Chinese Academy of Sciences
6.Institute of Hydrobiology, Chinese Academy of Sciences
7.Institute of Hydrobiology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang Binyuan,Zhai Gang,Gong Yulong,et al. Depletion of insulin receptors leads to b-cell hyperplasia in zebrafish[J]. Science Bulletin,2017,62(7):486.
APA Yang Binyuan.,Zhai Gang.,Gong Yulong.,Su Jingzhi.,Han Dong.,...&Xie Shouqi.(2017).Depletion of insulin receptors leads to b-cell hyperplasia in zebrafish.Science Bulletin,62(7),486.
MLA Yang Binyuan,et al."Depletion of insulin receptors leads to b-cell hyperplasia in zebrafish".Science Bulletin 62.7(2017):486.
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