IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China
Wang, Ping1,2; Jie, Li3; He, Tian Tian2; Li, Nan2; Mo, Zhao Lan3; Nie, Pin1,2,4; Xie, Hai Xia2
Corresponding AuthorNie, Pin(pinnie@ihb.ac.cn) ; Xie, Hai Xia(xieh@ihb.ac.cn)
2020-07-27
Source PublicationJOURNAL OF FISH DISEASES
ISSN0140-7775
Pages10
AbstractAeromonas salmonicidais a gram-negative bacterium that is the causative agent of furunculosis. AnA. salmonicidastrain was isolated from diseased turbot (Scophthalmus maximus) with the sign of furunculosis from North China. Based onvapAgene, the strain was further classified asA. salmonicidasubsp.masoucidaRZ6S-1. Culturing RZ6S-1 strain at high temperature (28 degrees C) obtained the virulence attenuated strain RZ6S. Genome sequence comparison between the two strains revealed the loss of the type IV secretion system (T4SS) and type III secretion system (T3SS) from the native plasmid pAsmB-1 and pAsmC-1 of wild-type strain RZ6S-1, respectively. Further study demonstrated that the wild-type strain RZ6S-1, but not its derivative mutant RZ6S, can stimulate apoptosis. Elevated protein level of cleaved caspase-3 was detected from epithelioma papulosum cyprinid (EPC) cells infected with wild-type strain RZ6S-1 as compared with that infected with RZ6S strain. Meanwhile, the invasion of the mutant strain RZ6S was about 17-fold higher than the wild-type strain RZ6S-1, suggesting that some protein(s) fromA. salmonicidasubsp.masoucidaRZ6S-1 suppress its invasion. The RZ6S mutant strain was attenuated, since its LD(50)is over 10,000 times higher compared to the wild-type strain as revealed in the turbot infection model.
KeywordAeromonas salmonicidasubsp masoucida apoptosis identification invasion virulence
DOI10.1111/jfd.13224
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[U1706205] ; Agriculture Research System of China[CARS-46]
WOS Research AreaFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000552556400001
WOS KeywordATYPICAL AEROMONAS-SALMONICIDA ; III SECRETION SYSTEM ; SUBSP-SALMONICIDA ; EFFECTOR PROTEIN ; VIRULENCE ; INFECTION ; GENES
PublisherWILEY
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China ; Agriculture Research System of China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36883
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, Pin; Xie, Hai Xia
Affiliation1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
3.Chinese Acad Fishery Sci, Key Lab Maricultural Organism Dis Control, Minist Agr & Rural Affairs, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ping,Jie, Li,He, Tian Tian,et al. Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China[J]. JOURNAL OF FISH DISEASES,2020:10.
APA Wang, Ping.,Jie, Li.,He, Tian Tian.,Li, Nan.,Mo, Zhao Lan.,...&Xie, Hai Xia.(2020).Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China.JOURNAL OF FISH DISEASES,10.
MLA Wang, Ping,et al."Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China".JOURNAL OF FISH DISEASES (2020):10.
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