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Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8
He, Tian Tian1,2; Zhou, Ying1,2; Liu, Ying Li1,2; Li, Duan You1,2; Nie, Pin1,3,4; Li, Ai Hua1; Xie, Hai Xia1
Corresponding AuthorLi, Ai Hua(liaihua@ihb.ac.cn) ; Xie, Hai Xia(xieh@ihb.ac.cn)
2020-07-01
Source PublicationCELLULAR MICROBIOLOGY
ISSN1462-5814
Volume22Issue:7Pages:16
AbstractThe type III secretion system effector EseJ plays a regulatory role inside bacteria. It suppresses the adherence of Edwardsiella piscicida (E. piscicida) to host epithelial cells by down regulating type 1 fimbriae. In this study, we observed that more macrophages infected with Delta eseJ strain of E. piscicida detached as compared with those infected with the wild-type (WT) strain. Terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) staining and cleaved caspase-3 examination revealed that the detachment is due to increased apoptosis, suggesting that EseJ suppresses macrophage apoptosis. However, apoptosis inhibition by EseJ is not relative to a type III secretion system (T3SS) and is not related to EseJ's translocation. Since EseJ negatively regulates type 1 fimbriae, murine J774A.1 cells were infected with Delta eseJ Delta fimA or Delta eseJ Delta fimH strains. It was demonstrated that Delta eseJ stimulates macrophage apoptosis through type 1 fimbriae. Moreover, we found that infecting J774A.1 cells with the Delta eseJ strain increased levels of cleaved caspase-8, caspase-9, and caspase-3, demonstrating that EseJ inhibits apoptosis through either an extrinsic or a combination of extrinsic and intrinsic pathways. Pre-treatment of macrophages with caspase-8 inhibitor prior to infection with the Delta eseJ strain decreased the levels of cleaved caspase-8, caspase-9, and caspase-3, indicating that the Delta eseJ strain stimulates apoptosis, mainly through an extrinsic pathway by up regulating type 1 fimbriae. Zebrafish larvae or blue gourami fish infected with the Delta eseJ strain consistently exhibited higher apoptosis than those infected with the E. piscicida WT strain or Delta eseJ Delta fimA strain. Taken together, we revealed that the T3SS protein EseJ of E. piscicida inhibits host apoptosis, mainly through an extrinsic pathway by down regulating type 1 fimbriae.
Keywordapoptosis Edwardsiella piscicida type 1 fimbriae type III protein EseJ
DOI10.1111/cmi.13193
Indexed BySCI ; SCI
Language英语
WOS Research AreaCell Biology ; Microbiology
WOS SubjectCell Biology ; Microbiology
WOS IDWOS:000540798600006
WOS KeywordSECRETION SYSTEM ; CHROMOSOMAL GENES ; CELL-DEATH ; TARDA ; IDENTIFICATION ; EFFECTOR ; ACTIVATION ; CLUSTER
PublisherWILEY
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36818
Collection中科院水生所知识产出(2009年前)
Corresponding AuthorLi, Ai Hua; Xie, Hai Xia
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
He, Tian Tian,Zhou, Ying,Liu, Ying Li,et al. Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8[J]. CELLULAR MICROBIOLOGY,2020,22(7):16.
APA He, Tian Tian.,Zhou, Ying.,Liu, Ying Li.,Li, Duan You.,Nie, Pin.,...&Xie, Hai Xia.(2020).Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8.CELLULAR MICROBIOLOGY,22(7),16.
MLA He, Tian Tian,et al."Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8".CELLULAR MICROBIOLOGY 22.7(2020):16.
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