IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Transcriptomic profiling revealed key signaling pathways for cold tolerance and acclimation of two carp species
Ge,Guodong1,2,4; Long,Yong1; Shi,Lianyu3; Ren,Jing1,2; Yan,Junjun1,2; Li,Chitao3; Li,Qing1; Cui,Zongbin1,4
2020-08-05
Source PublicationBMC Genomics
Volume21Issue:1Pages:1-15
Abstract

AbstractBackgroundClosely related species of the carp family (Cyprinidae) have evolved distinctive abilities to survive under cold stress, but molecular mechanisms underlying the generation of cold resistance remain largely unknown. In this study, we compared transcriptomic profiles of two carp species to identify key factors and pathways for cold tolerance and acclimation.ResultsLarvae of Songpu mirror carp and Barbless carp that were pretreated at 18?°C for 24?h significantly improved their survival rates under lethal cold temperature at 8?°C or 10?°C, indicating that two carp species possess the ability of cold acclimation. However, Songpu mirror carp exhibited stronger abilities of cold tolerance and acclimation than Barbless carp. Transcriptomic profiles of Songpu mirror carp and Barbless carp larvae at 28?°C and 18?°C were compared during cold acclimation through RNA-seq. Differentially expressed genes that are closely associated with the differences in cold acclimation between two carp species were identified through bioinformatics and Venn’s diagram analysis. GO enrichment analysis of these genes indicated that cellular component assembly involved in morphogenesis, secondary alcohol metabolism and drug transport were the most up-regulated biological processes during cold acclimation of Songpu mirror carp. Conversely, positive regulation of macroautophagy, intracellular protein transport, and organonitrogen compound catabolism were the most down-regulated biological processes during cold acclimation of Barbless carp. KEGG enrichment analysis revealed that factors in the FoxO-related signaling pathways are mainly responsible for the development of differences in cold tolerance and acclimation between two carp species since altering the phosphorylation of key proteins in the FoxO-related signaling pathways with inhibitors or an activator significantly decreased the cold tolerance and acclimation of Songpu mirror carp. These data provided key clues for dissection of molecular mechanisms underlying the development of cold tolerance and acclimation in carps.ConclusionsThese findings indicate that larvae of two carp species possess different abilities of cold tolerance and can build cold acclimation under mild low temperature. Multiple biological processes and FoxO-related signaling pathways are closely associated with the development of differences in cold tolerance and acclimation between two carp species.

KeywordCarp Cold tolerance Cold acclimation RNA-Seq Biological process Signaling pathways
DOI10.1186/s12864-020-06946-8
Language英语
WOS IDBMC:10.1186/s12864-020-06946-8
PublisherBioMed Central
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36775
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorLong,Yong; Cui,Zongbin
Affiliation1.Institute of Hydrobiology, Chinese Academy of Sciences; State Key Laboratory of Freshwater Ecology and Biotechnology
2.University of Chinese Academy of Sciences
3.Heilongjiang River Fishery Research Institute of Chinese Academy of Fishery Sciences
4.Guangdong Institute of Microbiology, Guangdong Academy of Sciences; State Key Laboratory of Applied Microbiology Southern China
Recommended Citation
GB/T 7714
Ge,Guodong,Long,Yong,Shi,Lianyu,et al. Transcriptomic profiling revealed key signaling pathways for cold tolerance and acclimation of two carp species[J]. BMC Genomics,2020,21(1):1-15.
APA Ge,Guodong.,Long,Yong.,Shi,Lianyu.,Ren,Jing.,Yan,Junjun.,...&Cui,Zongbin.(2020).Transcriptomic profiling revealed key signaling pathways for cold tolerance and acclimation of two carp species.BMC Genomics,21(1),1-15.
MLA Ge,Guodong,et al."Transcriptomic profiling revealed key signaling pathways for cold tolerance and acclimation of two carp species".BMC Genomics 21.1(2020):1-15.
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