| Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae) | |
Li, Gaojie1,2; Hu, Shiqi1,2; Yang, Jingjing1,2; Zhao, Xuyao1,2; Kimura, Seisuke3,4; Schultz, Elizabeth A.5; Hou, Hongwei1,2
| |
| Corresponding Author | Hou, Hongwei(houhw@ihb.ac.cn) |
| 2020-06-01 | |
| Source Publication | PLANT CELL REPORTS
 |
| ISSN | 0721-7714 |
| Volume | 39Issue:6Pages:737-750 |
| Abstract | Key message This is the first report of a highly efficient Agrobacterium tumefaciens-mediated transformation protocol for Acanthaceae and its utilization in revealing important roles of cytokinin in regulating heterophylly in Hygrophila difformis. Plants show amazing morphological differences in leaf form in response to changes in the surrounding environment, which is a phenomenon called heterophylly. Previous studies have shown that the aquatic plant Hygrophila difformis (Acanthaceae) is an ideal model for heterophylly study. However, low efficiency and poor reproducibility of genetic transformation restricted H. difformis as a model plant. In this study, we reported successful induction of callus, shoots and the establishment of an efficient stable transformation protocol as mediated by Agrobacterium tumefaciens LBA4404. We found that the highest callus induction efficiency was achieved with 1 mg/L 1-Naphthaleneacetic acid (NAA) and 2 mg/L 6-benzyladenine (6-BA), that efficient shoot induction required 0.1 mg/L NAA and 0.1 mg/L 6-BA and that high transformation efficiency required 100 mu M acetosyringone. Due to the importance of phytohormones in the regulation of heterophylly and the inadequate knowledge about the function of cytokinin (CK) in this process, we analyzed the function of CK in the regulation of heterophylly by exogenous CK application and endogenous CK detection. By using our newly developed transformation system to detect CK signals, contents and distribution in H. difformis, we revealed an important role of CK in environmental mediated heterophylly. |
| Keyword | Agrobacterium tumefaciens Cytokinin Heterophylly Hygrophila difformis Transformation Leaf form |
| DOI | 10.1007/s00299-020-02527-x |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| WOS Research Area | Plant Sciences |
| WOS Subject | Plant Sciences |
| WOS ID | WOS:000533794600005 |
| WOS Keyword | CARBON-DIOXIDE ; ABSCISIC-ACID ; LEAF ; INDUCTION ; MORPHOGENESIS ; GIBBERELLIN ; MECHANISMS ; ETHYLENE ; EXPLANTS ; GROWTH |
| Publisher | SPRINGER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/36620 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Corresponding Author | Hou, Hongwei |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Kyoto Sangyo Univ, Fac Life Sci, Kita Ku, Kyoto, Kyoto 6038555, Japan 4.Kyoto Sangyo Univ, Ctr Ecol Evolutionary Dev Biol, Kita Ku, Kyoto, Kyoto 6038555, Japan 5.Univ Lethbridge, Dept Biol Sci, Lethbridge, AB T1K 3M4, Canada |
| Recommended Citation GB/T 7714 | Li, Gaojie,Hu, Shiqi,Yang, Jingjing,et al. Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae)[J]. PLANT CELL REPORTS,2020,39(6):737-750. |
| APA | Li, Gaojie.,Hu, Shiqi.,Yang, Jingjing.,Zhao, Xuyao.,Kimura, Seisuke.,...&Hou, Hongwei.(2020).Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae).PLANT CELL REPORTS,39(6),737-750. |
| MLA | Li, Gaojie,et al."Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae)".PLANT CELL REPORTS 39.6(2020):737-750. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment