IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae)
Li, Gaojie1,2; Hu, Shiqi1,2; Yang, Jingjing1,2; Zhao, Xuyao1,2; Kimura, Seisuke3,4; Schultz, Elizabeth A.5; Hou, Hongwei1,2
Corresponding AuthorHou, Hongwei(houhw@ihb.ac.cn)
2020-06-01
Source PublicationPLANT CELL REPORTS
ISSN0721-7714
Volume39Issue:6Pages:737-750
AbstractKey message This is the first report of a highly efficient Agrobacterium tumefaciens-mediated transformation protocol for Acanthaceae and its utilization in revealing important roles of cytokinin in regulating heterophylly in Hygrophila difformis. Plants show amazing morphological differences in leaf form in response to changes in the surrounding environment, which is a phenomenon called heterophylly. Previous studies have shown that the aquatic plant Hygrophila difformis (Acanthaceae) is an ideal model for heterophylly study. However, low efficiency and poor reproducibility of genetic transformation restricted H. difformis as a model plant. In this study, we reported successful induction of callus, shoots and the establishment of an efficient stable transformation protocol as mediated by Agrobacterium tumefaciens LBA4404. We found that the highest callus induction efficiency was achieved with 1 mg/L 1-Naphthaleneacetic acid (NAA) and 2 mg/L 6-benzyladenine (6-BA), that efficient shoot induction required 0.1 mg/L NAA and 0.1 mg/L 6-BA and that high transformation efficiency required 100 mu M acetosyringone. Due to the importance of phytohormones in the regulation of heterophylly and the inadequate knowledge about the function of cytokinin (CK) in this process, we analyzed the function of CK in the regulation of heterophylly by exogenous CK application and endogenous CK detection. By using our newly developed transformation system to detect CK signals, contents and distribution in H. difformis, we revealed an important role of CK in environmental mediated heterophylly.
KeywordAgrobacterium tumefaciens Cytokinin Heterophylly Hygrophila difformis Transformation Leaf form
DOI10.1007/s00299-020-02527-x
Indexed BySCI ; SCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000533794600005
WOS KeywordCARBON-DIOXIDE ; ABSCISIC-ACID ; LEAF ; INDUCTION ; MORPHOGENESIS ; GIBBERELLIN ; MECHANISMS ; ETHYLENE ; EXPLANTS ; GROWTH
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36620
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorHou, Hongwei
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Kyoto Sangyo Univ, Fac Life Sci, Kita Ku, Kyoto, Kyoto 6038555, Japan
4.Kyoto Sangyo Univ, Ctr Ecol Evolutionary Dev Biol, Kita Ku, Kyoto, Kyoto 6038555, Japan
5.Univ Lethbridge, Dept Biol Sci, Lethbridge, AB T1K 3M4, Canada
Recommended Citation
GB/T 7714
Li, Gaojie,Hu, Shiqi,Yang, Jingjing,et al. Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae)[J]. PLANT CELL REPORTS,2020,39(6):737-750.
APA Li, Gaojie.,Hu, Shiqi.,Yang, Jingjing.,Zhao, Xuyao.,Kimura, Seisuke.,...&Hou, Hongwei.(2020).Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae).PLANT CELL REPORTS,39(6),737-750.
MLA Li, Gaojie,et al."Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae)".PLANT CELL REPORTS 39.6(2020):737-750.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Gaojie]'s Articles
[Hu, Shiqi]'s Articles
[Yang, Jingjing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Gaojie]'s Articles
[Hu, Shiqi]'s Articles
[Yang, Jingjing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Gaojie]'s Articles
[Hu, Shiqi]'s Articles
[Yang, Jingjing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.