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Analysis of Multiplicity of Hypoxia-Inducible Factors in the Evolution of Triplophysa Fish (Osteichthyes: Nemacheilinae) Reveals Hypoxic Environments Adaptation to Tibetan Plateau
Chen, Juan1,2; Shen, Yanjun1,2; Wang, Jing3; Ouyang, Gang3; Kang, Jingliang1,2; Lv, Wenqi1,2; Yang, Liandong1; He, Shunping1,4
Corresponding AuthorYang, Liandong(yangliandong1987@163.com) ; He, Shunping(clad@ihb.ac.cn)
2020-05-12
Source PublicationFRONTIERS IN GENETICS
Volume11Pages:15
AbstractHIF (Hypoxia-inducible factor) gene family members function as master regulators of cellular and systemic oxygen homeostasis during changes in oxygen availability. Qinghai-Tibet Plateau is a natural laboratory for for long-term hypoxia and cold adaptation. In this context, T. scleroptera that is restricted to >3500 m high-altitude freshwater rivers was selected as the model to compare with a representative species from the plain, P. dabryanus. We cloned different HIF-alpha and carried out a phylogenetic analysis from invertebrates to vertebrates for identifying HIF-alpha genes and analyzing their evolutionary history. Intriguingly, the HIF-alpha has undergone gene duplications might be due to whole-genome duplication (WGD) events during evolution. PAML analysis indicated that HIF-1 alpha A was subjected to positive selection acted on specific sites in Triplophysa lineages. To investigate the relationship between hypoxia adaptation and the regulation of HIF-alpha stability by pVHL in plateau and plain fish, a series of experiments were carried out. Comparison the luciferase transcriptional activity and protein levels of HIF-alpha s and the differing interactions of HIF-alpha s with pVHL, show clear differences between plateau and plain fish. T. scleroptera pVHL could enhance HIF-alpha transcriptional activity under hypoxia, and functional validation through pVHL protein mutagenesis showed that these mutations increased the stability of HIF-alpha and its hetero dimerization affinity to ARNT. Our research shows that missense mutations of pVHL induced evolutionary molecular adaptation in Triplophysa fishes living in high altitude hypoxic environments.
KeywordHIF-alpha pVHL the Tibetan Plateau hypoxia adaption Triplophysa fish
DOI10.3389/fgene.2020.00433
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31601858] ; National Natural Science Foundation of China[31972866] ; National Natural Science Foundation of China[91731301] ; Strategic Priority Research Program[XDB13020100] ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000537193400001
WOS KeywordHIGH-ALTITUDE ADAPTATION ; FACTOR-I ; TRANSCRIPTOME ANALYSIS ; GENOME DUPLICATION ; HIF-ALPHA ; TOLERANCE ; HIF-1-ALPHA ; HIF-2-ALPHA ; QINGHAI ; PROTEIN
PublisherFRONTIERS MEDIA SA
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Strategic Priority Research Program ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China ; Wuhan Branch, Supercomputing Center, Chinese Academy of Sciences, China
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36552
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorYang, Liandong; He, Shunping
Affiliation1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat Chinese Acad, Inst Hydrobiol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China
4.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Chen, Juan,Shen, Yanjun,Wang, Jing,et al. Analysis of Multiplicity of Hypoxia-Inducible Factors in the Evolution of Triplophysa Fish (Osteichthyes: Nemacheilinae) Reveals Hypoxic Environments Adaptation to Tibetan Plateau[J]. FRONTIERS IN GENETICS,2020,11:15.
APA Chen, Juan.,Shen, Yanjun.,Wang, Jing.,Ouyang, Gang.,Kang, Jingliang.,...&He, Shunping.(2020).Analysis of Multiplicity of Hypoxia-Inducible Factors in the Evolution of Triplophysa Fish (Osteichthyes: Nemacheilinae) Reveals Hypoxic Environments Adaptation to Tibetan Plateau.FRONTIERS IN GENETICS,11,15.
MLA Chen, Juan,et al."Analysis of Multiplicity of Hypoxia-Inducible Factors in the Evolution of Triplophysa Fish (Osteichthyes: Nemacheilinae) Reveals Hypoxic Environments Adaptation to Tibetan Plateau".FRONTIERS IN GENETICS 11(2020):15.
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