IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila
Maa, Yang1,2,3; Yen, Guanxiong1,2,3; Han, Xiaojie2,4; Zhang, Jing2; Xiong, Jie2; Miao, Wei1,2,3,5
Corresponding AuthorMiao, Wei(miaowei@ihb.ac.cn)
2020-03-01
Source PublicationJOURNAL OF CELL SCIENCE
ISSN0021-9533
Volume133Issue:6Pages:12
AbstractTo investigate the mechanisms underlying initiation of the sexual cell cycle in eukaryotes, we have focused on cyclins and cyclin-dependent kinases (CDKs) in the well-studied model ciliate, Tetrahymena thermophila. We identified two genes, CDK19 and CYC9, which are highly co-expressed with the mating-associated factors MTA, MTB and HAP2. Both CDK19 and CYC9 were found to be essential for mating in T. thermophila. Subcellular localization experiments suggested that these proteins are located at the oral area, including the conjugation junction area, and that CDK19 or CYC9 knockout prevents mating. We found that CDK19 and CYC9 form a complex, and also identified several additional subunits, which may have regulatory or constitutive functions. RNA sequencing analyses and cytological experiments showed that mating is abnormal in both Delta CDK19 and Delta CYC9, mainly at the entry to the co-stimulation stage. These results indicate that the CDK19-CYC9 complex initiates the sexual cell cycle in T. thermophila.
KeywordCell cycle Cyclin Cyclin-dependent kinase Sexual reproduction Tetrahymena thermophila
DOI10.1242/jcs.235721
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[91631303] ; National Natural Science Foundation of China[31525021]
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000534387000010
WOS KeywordDEVELOPMENTAL INTERACTION ; DEPENDENT KINASE ; CO-STIMULATION ; PAIR FORMATION ; GENE ONTOLOGY ; PROTEIN ; TRANSFORMATION ; CONJUGATION ; MEIOSIS ; CLASSIFICATION
PublisherCOMPANY BIOLOGISTS LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36535
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorMiao, Wei
Affiliation1.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodiversily & Conservat, Wuhan 430072, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
5.CAS Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Maa, Yang,Yen, Guanxiong,Han, Xiaojie,et al. Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila[J]. JOURNAL OF CELL SCIENCE,2020,133(6):12.
APA Maa, Yang,Yen, Guanxiong,Han, Xiaojie,Zhang, Jing,Xiong, Jie,&Miao, Wei.(2020).Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila.JOURNAL OF CELL SCIENCE,133(6),12.
MLA Maa, Yang,et al."Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila".JOURNAL OF CELL SCIENCE 133.6(2020):12.
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