| Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila | |
| Maa, Yang1,2,3; Yen, Guanxiong1,2,3; Han, Xiaojie2,4; Zhang, Jing2; Xiong, Jie2; Miao, Wei1,2,3,5 | |
| Corresponding Author | Miao, Wei(miaowei@ihb.ac.cn) |
| 2020-03-01 | |
| Source Publication | JOURNAL OF CELL SCIENCE
 |
| ISSN | 0021-9533 |
| Volume | 133Issue:6Pages:12 |
| Abstract | To investigate the mechanisms underlying initiation of the sexual cell cycle in eukaryotes, we have focused on cyclins and cyclin-dependent kinases (CDKs) in the well-studied model ciliate, Tetrahymena thermophila. We identified two genes, CDK19 and CYC9, which are highly co-expressed with the mating-associated factors MTA, MTB and HAP2. Both CDK19 and CYC9 were found to be essential for mating in T. thermophila. Subcellular localization experiments suggested that these proteins are located at the oral area, including the conjugation junction area, and that CDK19 or CYC9 knockout prevents mating. We found that CDK19 and CYC9 form a complex, and also identified several additional subunits, which may have regulatory or constitutive functions. RNA sequencing analyses and cytological experiments showed that mating is abnormal in both Delta CDK19 and Delta CYC9, mainly at the entry to the co-stimulation stage. These results indicate that the CDK19-CYC9 complex initiates the sexual cell cycle in T. thermophila. |
| Keyword | Cell cycle Cyclin Cyclin-dependent kinase Sexual reproduction Tetrahymena thermophila |
| DOI | 10.1242/jcs.235721 |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[91631303] ; National Natural Science Foundation of China[31525021] |
| WOS Research Area | Cell Biology |
| WOS Subject | Cell Biology |
| WOS ID | WOS:000534387000010 |
| WOS Keyword | DEVELOPMENTAL INTERACTION ; DEPENDENT KINASE ; CO-STIMULATION ; PAIR FORMATION ; GENE ONTOLOGY ; PROTEIN ; TRANSFORMATION ; CONJUGATION ; MEIOSIS ; CLASSIFICATION |
| Publisher | COMPANY BIOLOGISTS LTD |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/36535 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Corresponding Author | Miao, Wei |
| Affiliation | 1.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodiversily & Conservat, Wuhan 430072, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China 5.CAS Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China |
| Recommended Citation GB/T 7714 | Maa, Yang,Yen, Guanxiong,Han, Xiaojie,et al. Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila[J]. JOURNAL OF CELL SCIENCE,2020,133(6):12. |
| APA | Maa, Yang,Yen, Guanxiong,Han, Xiaojie,Zhang, Jing,Xiong, Jie,&Miao, Wei.(2020).Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila.JOURNAL OF CELL SCIENCE,133(6),12. |
| MLA | Maa, Yang,et al."Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila".JOURNAL OF CELL SCIENCE 133.6(2020):12. |
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