IHB OpenIR  > 水环境工程研究中心  > 期刊论文
Study on the safe disposal and resource utilization of cyanobacterial bloom biomass in Dianchi Lake, China
Shen, Qiang1; Li, Dewang1; Li, Dunhai2; Liu, Yongding2; Li, Jianyong1; Li, Sixin1
Corresponding AuthorShen, Qiang(firefox1111@163.com)
2020-04-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Volume32Issue:2Pages:1201-1213
AbstractTo solve the problem of utilizing massive harmful algal blooms (HABs) biomass, we developed a technique involving physical purification methods, namely, microfiltration (2 mu m, 0.45 mu m), ultrafiltration (100 kDa cutoff), and low-temperature precipitation (4 degrees C, 48 h), as the core methodology and used the toxic Microcystis biomass in Dianchi Lake for batch preparations of microcystins (MCs) and phycocyanin (PC). The results were as follows: (1) From 1.0 kg of Dianchi Lake cyanobacteria, 57 g of PC powder with a purity (A(620)/A(280)) of 1.78 was prepared. An acute oral toxicity test in mice showed that the LD50 of the prepared PC was >5.25 g kg(-1), practically non-toxic. The LD50 of PC administered by intraperitoneal injection was >4.71 g kg(-1). The Ames test showed that the mutagenic effect was negative independent of the addition of S9. The overall results of the toxicity tests suggested that the prepared PC was not potentially toxic. (2) From 666.7 g of cyanobacteria, 2.262 g of MC extract powder (containing 192.7 mg of MC-RR and 54.3 mg of MC-LR) was prepared. MC-RR and MC-LR accounted for 8.52% and 2.40%, respectively, and the extraction ratios for MC-RR and MC-LR were 40.1% and 83.1%, respectively. A further purification by preparative HPLC was carried out, obtaining 2.338 mg of pure MC-RR (chromatographic purity >85%) from 70 mg of MC extract powder, which was used as an HPLC chromatographic standard and in routine toxicology experiments. The efficiency of the ultrafiltration treatment of cyanobacterial powder reached 49.3 kg d(-1), and the cost of producing food-grade PC powder in this study was 17.6 US$ g(-1), which is only 13.6% of the current price of food-grade PC on the international market. Thus, the cost advantage was significant. Therefore, this study provides an approach for the safe disposal of HABs and the large-scale commercial utilization of HAB biomass.
KeywordHarmful algae blooms Phycocyanin Microcystin Ultrafiltration Low-temperature precipitation Dianchi Lake
DOI10.1007/s10811-019-01995-3
Indexed BySCI ; SCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000534338500037
WOS KeywordC-PHYCOCYANIN ; PURIFICATION ; EXTRACTION ; TAIHU ; TOXICITY ; MICROCYSTINS ; CYANOTOXINS ; MECHANISMS ; DIVERSITY ; PLATENSIS
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36529
Collection水环境工程研究中心_期刊论文
Corresponding AuthorShen, Qiang
Affiliation1.Minist Water Resources & Chinese Acad Sci, Inst Hydroecol, Key Lab Ecol Impacts Hydraul Projects & Restorat, Wuhan 430079, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Shen, Qiang,Li, Dewang,Li, Dunhai,et al. Study on the safe disposal and resource utilization of cyanobacterial bloom biomass in Dianchi Lake, China[J]. JOURNAL OF APPLIED PHYCOLOGY,2020,32(2):1201-1213.
APA Shen, Qiang,Li, Dewang,Li, Dunhai,Liu, Yongding,Li, Jianyong,&Li, Sixin.(2020).Study on the safe disposal and resource utilization of cyanobacterial bloom biomass in Dianchi Lake, China.JOURNAL OF APPLIED PHYCOLOGY,32(2),1201-1213.
MLA Shen, Qiang,et al."Study on the safe disposal and resource utilization of cyanobacterial bloom biomass in Dianchi Lake, China".JOURNAL OF APPLIED PHYCOLOGY 32.2(2020):1201-1213.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shen, Qiang]'s Articles
[Li, Dewang]'s Articles
[Li, Dunhai]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shen, Qiang]'s Articles
[Li, Dewang]'s Articles
[Li, Dunhai]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shen, Qiang]'s Articles
[Li, Dewang]'s Articles
[Li, Dunhai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.