| De novo transcriptome assembly of four organs of Collichthys lucidus and identification of genes involved in sex determination and reproduction | |
| Song, Wei1,2; Zhang, YiBing1; Zhang, XiaoJuan1; Gui, JianFang1 | |
| Corresponding Author | Gui, JianFang(jfgui@ihb.ac.cn) |
| 2020-03-27 | |
| Source Publication | PLOS ONE
 |
| ISSN | 1932-6203 |
| Volume | 15Issue:3Pages:16 |
| Abstract | The spinyhead croaker (Collichthys lucidus) is a commercially important fish species, which is mainly distributed in the coastal regions of China. However, little is known about the molecular regulatory mechanism underlying reproduction in C. lucidus. A de novo transcriptome assembly in brain, liver, ovary and testis tissues of C. lucidus was performed. Illumina sequencing generated 60,322,004, 57,044,284, 60,867,978 and 57,087,688 clean reads from brain, liver, ovary and testis tissues of C. lucidus, respectively. Totally, 131,168 unigenes with an average length of 644 bp and an N50 value of 1033 bp were assembled. In addition, 1288 genes were differentially expressed between ovary and testis, including 442 up-regulated and 846 down-regulated in ovary. Functional analysis revealed that the differentially expressed genes between ovary and testis were mainly involved in the function of sexual reproduction, sex differentiation, development of primary male sexual characteristics, female gamete generation, and male sex differentiation. A number of genes which might be involved in the regulation of reproduction and sex determination were found, including HYAL and SYCP3 and BMP15. Furthermore, 35,476 simple sequence repeats (SSRs) were identified in this transcriptome dataset, which would contribute to further genetic and mechanism researches. De novo transcriptome sequencing analysis of four organs of C. lucidus provides rich resources for understanding the mechanism of reproductive development of C. lucidus and further investigation of the molecular regulation of sex determination and reproduction of C. lucidus. |
| DOI | 10.1371/journal.pone.0230580 |
| Funding Organization | Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS[Dong2018Z01] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000535935400015 |
| WOS Keyword | PITUITARY-GONADAL AXIS ; SPINYHEAD CROAKER ; ADRENAL AXES ; EXPRESSION ; ZEBRAFISH ; RECEPTOR ; CATFISH ; DIFFERENTIATION ; IMPROVEMENT ; PROFILES |
| Publisher | PUBLIC LIBRARY SCIENCE |
| Funding Organization | Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS ; Basic Research Fund for State-Level Nonprofit Research Institutes of ESCFRI, CAFS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/36501 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Gui, JianFang |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China 2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai, Peoples R China |
| Recommended Citation GB/T 7714 | Song, Wei,Zhang, YiBing,Zhang, XiaoJuan,et al. De novo transcriptome assembly of four organs of Collichthys lucidus and identification of genes involved in sex determination and reproduction[J]. PLOS ONE,2020,15(3):16. |
| APA | Song, Wei,Zhang, YiBing,Zhang, XiaoJuan,&Gui, JianFang.(2020).De novo transcriptome assembly of four organs of Collichthys lucidus and identification of genes involved in sex determination and reproduction.PLOS ONE,15(3),16. |
| MLA | Song, Wei,et al."De novo transcriptome assembly of four organs of Collichthys lucidus and identification of genes involved in sex determination and reproduction".PLOS ONE 15.3(2020):16. |
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