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Morphological description of Opalina obtrigonoidea Metcalf, 1923 (Heterokonta, Opalinea) from Duttaphrynus melanostictus and evaluation of the ITS region as a suitable genetic marker for inter-species identification in Opalina
Zhao, Weishan1,2,3; Hu, Guangran1,2,3; Ponce-Gordo, Francisco4; Zou, Hong1,2; Li, Wenxiang1,2; Wu, Shangong1,2; Li, Ming1,2; Wang, Guitang1,2
Corresponding AuthorLi, Ming(liming@ihb.ac.cn)
2020-06-01
Source PublicationPARASITOLOGY INTERNATIONAL
ISSN1383-5769
Volume76Pages:7
AbstractThe redescription of Opalina obtrigonoidea Metcalf, 1923, collected from the rectum of the toads Duttaphrynus melanostictus, is presented in this paper based on detailed morphological information and molecular data. Our results revealed that O. obtrigonoidea varies greatly in body dimensions. Its morphological characteristics allow its differentiation from Opalina undulata. Surprisingly, we sequenced its SSU rDNA-ITS1-5.8S rDNA-ITS2-LSU rDNA (5' end) and found the SSU rDNA of O. obtrigonoidea is nearly identical to that of O. undulata. However, there are differences in both the ITS1 and ITS2 regions that allow their distinction and confirm the morphological differences. Our results indicate that O. obtrigonoidea and O. undulata are closely related species in which morphological and genetic markers have evolved at different speeds. Due to this, the SSU rDNA gene may not be a valid marker for inter-species identification in Opalina, but the ITS is a valid marker for differentiating species in this genus.
KeywordOpalina obtrigonoidea Duttaphrynus melanostictus Morphology SSU rDNA ITS region
DOI10.1016/j.parint.2020.102103
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31772429] ; Youth Innovation Promotion Association CAS[Y82Z01] ; second Tibetan Plateau Scientific Expedition and Research Program (STEP)[2019QZKK0304] ; earmarked fund for China Agriculture Research System[CARS-45-15]
WOS Research AreaParasitology
WOS SubjectParasitology
WOS IDWOS:000528164100045
WOS KeywordCLUSTAL-W ; ITS2 ; PROTOZOA
PublisherELSEVIER IRELAND LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36417
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLi, Ming
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Minist Agr, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Univ Complutense Madrid, Fac Farm, Dept Microbiol & Parasitol, Plaza Ramony Cajal S-N, E-28040 Madrid, Spain
Recommended Citation
GB/T 7714
Zhao, Weishan,Hu, Guangran,Ponce-Gordo, Francisco,et al. Morphological description of Opalina obtrigonoidea Metcalf, 1923 (Heterokonta, Opalinea) from Duttaphrynus melanostictus and evaluation of the ITS region as a suitable genetic marker for inter-species identification in Opalina[J]. PARASITOLOGY INTERNATIONAL,2020,76:7.
APA Zhao, Weishan.,Hu, Guangran.,Ponce-Gordo, Francisco.,Zou, Hong.,Li, Wenxiang.,...&Wang, Guitang.(2020).Morphological description of Opalina obtrigonoidea Metcalf, 1923 (Heterokonta, Opalinea) from Duttaphrynus melanostictus and evaluation of the ITS region as a suitable genetic marker for inter-species identification in Opalina.PARASITOLOGY INTERNATIONAL,76,7.
MLA Zhao, Weishan,et al."Morphological description of Opalina obtrigonoidea Metcalf, 1923 (Heterokonta, Opalinea) from Duttaphrynus melanostictus and evaluation of the ITS region as a suitable genetic marker for inter-species identification in Opalina".PARASITOLOGY INTERNATIONAL 76(2020):7.
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