IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
Phylogenetic relationships and genetic divergence of paralytic shellfish toxin- and cylindrospermopsin- producing Cylindrospermopsis and raphidiopsis
Jiang, Yongguang1; Chen, Youxin2; Yang, Shimin1; Li, Renhui2
Corresponding AuthorLi, Renhui(reli@ihb.ac.cn)
2020-03-01
Source PublicationHARMFUL ALGAE
ISSN1568-9883
Volume93Pages:11
AbstractCylindrospermopsis and Raphidiopsis (C/R group) are closely related species responsible for cyanobacterial blooms worldwide. Paralytic shellfish toxins (PSTs) and cylindrospermopsins (CYNs) have been identified in different C/R group strains. However, the evolutionary relationship between PST- and CYN-producing strains has not been systematically evaluated. In this study, C/R group strains and their toxin biosynthesis genes were evaluated by phylogenetic analysis and sequence comparison. None of the tested strains are able to produce PSTs and CYNs simultaneously. The C/R group strains were clustered into five clades, including two non-toxic, two CYN-producing and one PST-producing clades. A high degree of similarity was observed for rpoC1 (> 96%) and ITS-L (> 97%) sequences within each clade with the exception of the ITS-L (87% to 100%) region in CYN-producing R. curvata, which has been shown to contain variable sequence insertions. Genomic analysis revealed that sxtY and sxtZ could be found in both toxic and non-toxic strains. The transposase gene IS4 was only observed in strains from the PST-producing clade. The sxt and cyr gene clusters share five gene families with similar functions. The amino acid sequences of the adenylyl-sulfate kinase genes, sxtO and cyrN, are more similar (45% to 81%) than other pairs of genes (8.0% to 40%). SxtO and CyrN proteins from C/R group strains forms an independent clade on the phylogenetic tree with a high degree of sequence similarity (78% to 100%). In conclusion, PST- and CYN-producing C/R group species can be classified into different clades based on their phylogenetic profile. The sxtO and cyrN genes have probably diverged from a single ancestral adenylyl-sulfate kinase gene, and may be specifically used for toxin biosynthesis in C/R group species.
KeywordCyanotoxin Saxitoxin Cylindrospermopsin Biosynthesis Gene cluster Phylogeny
DOI10.1016/j.hal.2020.101792
Funding OrganizationNational Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan)
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFA0901300] ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences[Y85Z061601] ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan)
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000527331100011
WOS KeywordCYANOBACTERIAL GENERA CYLINDROSPERMOPSIS ; RACIBORSKII ; BIOSYNTHESIS ; CLUSTER ; STRAIN ; PHYLOGEOGRAPHY ; NOSTOCALES ; DEOXYCYLINDROSPERMOPSIN ; DIVERSITY
PublisherELSEVIER
Funding OrganizationNational Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36411
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorLi, Renhui
Affiliation1.China Univ Geosci, Sch Environm Studies, Dept Biol Sci & Technol, Wuhan 430074, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Yongguang,Chen, Youxin,Yang, Shimin,et al. Phylogenetic relationships and genetic divergence of paralytic shellfish toxin- and cylindrospermopsin- producing Cylindrospermopsis and raphidiopsis[J]. HARMFUL ALGAE,2020,93:11.
APA Jiang, Yongguang,Chen, Youxin,Yang, Shimin,&Li, Renhui.(2020).Phylogenetic relationships and genetic divergence of paralytic shellfish toxin- and cylindrospermopsin- producing Cylindrospermopsis and raphidiopsis.HARMFUL ALGAE,93,11.
MLA Jiang, Yongguang,et al."Phylogenetic relationships and genetic divergence of paralytic shellfish toxin- and cylindrospermopsin- producing Cylindrospermopsis and raphidiopsis".HARMFUL ALGAE 93(2020):11.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jiang, Yongguang]'s Articles
[Chen, Youxin]'s Articles
[Yang, Shimin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jiang, Yongguang]'s Articles
[Chen, Youxin]'s Articles
[Yang, Shimin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jiang, Yongguang]'s Articles
[Chen, Youxin]'s Articles
[Yang, Shimin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.