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Sequencing and characterization of the macronuclear rDNA minichromosome of the protozoan Tetrahymena pyriformis
Han, Xiaojie1,2,3; Yan, Guanxiong2,3; Ma, Yang2,3; Miao, Wei1,2,3,4,5; Wang, Guangying2
2020-03-15
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume147Issue:1Pages:576-581
Abstract

Tetrahymena ribosomal DNA (rDNA) is an ideal system for studying eukaryotic DNA replication and gene transcription. In this study, we developed a new method to isolate rDNA from Tetrahymena cells and used it to sequence and annotate the complete 19,670 bp macronudear rDNA minichromosome of Tetrahymena pyriformis, a species that lacks the germ-line micronucleus and is unable to undergo sexual reproduction. The key features of T. pyriformis and Tetrahymena thermophila rDNA sequences were then compared. Our results showed (i) the short inverted repeats (M repeats) essential for formation of rDNA minichromosome palindromic structure during sexual reproduction in Tetrahymena are highly conserved in T. pyriformis; (ii) in contrast to T. thermophila. which has two tandem domains that coordinately regulate rDNA replication, T. pyriformis has only a single domain; (iii) the 35S pre-rRNA precursor has 8025% similarity between the two species; and (iv) the G + C content is higher in the transcribed region than the non-transcribed region in both species, but the GC-skew is more stable in T. pyriformis. The new isolation method and annotated information for the T. pyriformis rDNA minichromosome will provide a useful resource for studying DNA replication and chromosome copy number control in Tetrohymena. (C) 2020 Elsevier B.V. All rights reserved.

KeywordTetrahymena pyriformis rDNA minichromosome Palindrome
DOI10.1016/j.ijbiomac.2020.01.063
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000525864700062
WOS KeywordRIBOSOMAL-RNA GENES ; EXTRACHROMOSOMAL RDNA ; CHROMOSOME BREAKAGE ; DNA ; REPLICATION ; THERMOPHILA ; AMPLIFICATION
PublisherELSEVIER
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36080
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorWang, Guangying
Affiliation1.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
5.CAS Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Han, Xiaojie,Yan, Guanxiong,Ma, Yang,et al. Sequencing and characterization of the macronuclear rDNA minichromosome of the protozoan Tetrahymena pyriformis[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,147(1):576-581.
APA Han, Xiaojie,Yan, Guanxiong,Ma, Yang,Miao, Wei,&Wang, Guangying.(2020).Sequencing and characterization of the macronuclear rDNA minichromosome of the protozoan Tetrahymena pyriformis.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,147(1),576-581.
MLA Han, Xiaojie,et al."Sequencing and characterization of the macronuclear rDNA minichromosome of the protozoan Tetrahymena pyriformis".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 147.1(2020):576-581.
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