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Antioxidant-related catalase CTA1 regulates development, aflatoxin biosynthesis, and virulence in pathogenic fungus Aspergillus flavus
Zhu, Zhuo1; Yang, Mingkun1,2; Bai, Youhuang1; Ge, Feng2; Wang, Shihua1
2020-04-15
Source PublicationENVIRONMENTAL MICROBIOLOGY
ISSN1462-2912
Issue1Pages:19
Abstract

Reactive oxygen species (ROS) induce the synthesis of a myriad of secondary metabolites, including aflatoxins. It raises significant concern as it is a potent environmental contaminant. In Aspergillus flavus., antioxidant enzymes link ROS stress response with coordinated gene regulation of aflatoxin biosynthesis. In this study, we characterized the function of a core component of the antioxidant enzyme catalase (CTA1) of A. flavus. Firstly, we verified the presence of cta1 corresponding protein (CTA1) by Western blot analysis and mass-spectrometry based analysis. Then, the functional study revealed that the growth, sporulation and sclerotia formation significantly increased, while aflatoxins production and virulence were decreased in the cta1 deletion mutant as compared with the WT and complementary strains. Furthermore, the absence of the cta1 gene resulted in a significant rise in the intracellular ROS level, which in turn added to the oxidative stress level of cells. A further quantitative proteomics investigation hinted that in vivo, CTA1 might maintain the ROS level to facilitate the aflatoxin synthesis. All in all, the pleiotropic phenotype of A. flavus CTA1 deletion mutant revealed that the antioxidant system plays a crucial role in fungal development, aflatoxins biosynthesis and virulence.

DOI10.1111/1462-2920.15011
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000527009600001
WOS KeywordOXIDATIVE STRESS ; GENE-CLUSTER ; HYDROGEN-PEROXIDE ; DELETION ANALYSIS ; NIDULANS ; OXYGEN ; EXPRESSION ; PROTEIN ; INHIBITION ; OXYLIPINS
PublisherWILEY
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36037
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorWang, Shihua
Affiliation1.Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Fuzhou 350002, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Zhuo,Yang, Mingkun,Bai, Youhuang,et al. Antioxidant-related catalase CTA1 regulates development, aflatoxin biosynthesis, and virulence in pathogenic fungus Aspergillus flavus[J]. ENVIRONMENTAL MICROBIOLOGY,2020(1):19.
APA Zhu, Zhuo,Yang, Mingkun,Bai, Youhuang,Ge, Feng,&Wang, Shihua.(2020).Antioxidant-related catalase CTA1 regulates development, aflatoxin biosynthesis, and virulence in pathogenic fungus Aspergillus flavus.ENVIRONMENTAL MICROBIOLOGY(1),19.
MLA Zhu, Zhuo,et al."Antioxidant-related catalase CTA1 regulates development, aflatoxin biosynthesis, and virulence in pathogenic fungus Aspergillus flavus".ENVIRONMENTAL MICROBIOLOGY .1(2020):19.
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