IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
Dietary supplementation of Geotrichum candidum improves growth, gut microbiota, immune-related gene expression and disease resistance in gibel carp CAS III (Carassius auratus gibelio)
Noor-Ul, Huda1,2; Liu Haokun1; Jin Junyan1; Zhu Xiaoming1,3; Han Dong1,3; Yang Yunxia1; Xie Shouqi1,3
2020-04-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume99Issue:1Pages:144-153
Abstract

Geotrichum candidum is a filamentous fungus mostly used in the dairy industry for cheese ripening and flavoring. The current study was designed to evaluate the potential probiotics effect of dietary supplementation of G. candidum on growth, digestive enzymes activities, gut microbiota and immune-related gene expression and disease resistance in Gibel carp CAS III (Carassius auratus gibelio) against A. hydrophila infections. For this purpose, 420 healthy fish (initial body weight: 7.09 +/- 0.02g) were randomly divided into 7 feeding groups in triplicates of 20 fish in each tank. Seven isocaloric and isonitrogenous diets were prepared, supplemented with 6 different doses of G. candidum viz; C: 0, T1:10(6), T2:10(7), T3:10(8), T4:10(9), T5:10(10), T6:10(11) CFU/kg diet and fed to fish twice a day for 60 days. Results showed significantly higher weight gain (WG) and feed efficiency (FE) in groups fed 10(6) and 10(8) CFU/kg diet compared to the control (P < 0.05). All G. candidum fed groups showed higher intestinal a-amylase activity (P < 0.05) while no difference in lipase and trypsin activity compared to the control group. Before challenge test, gut microbial diversity analysis revealed relatively more abundance of bacteria belonging to phylum Proteobacteria, Actinobacteria, Firmicutes and Bacteriodetes in group fed G. candidum supplemented diet and higher percentage of pathogenic bacteria belonging to order Aeromonadales and Vibrionales in control post-challenge. After challenge test immune response expressed numerically higher survival rate (P > 0.05) and significantly higher (P < 0.05) Respiratory Burst Activity(RBA), Immunoglobulin M level (IgM), Aspartate Transaminase activity (AST) and improved (P > 0.05) lysozyme activity, and Alanine Transaminase activity (ALT) in groups fed 10(6) and 10(8) CFU/kg G. candidum supplemented diet. The expression of immune related gene (IL-I beta, TNF- alpha, HSP70 and TLR-2) in liver of the fish were significantly affected (P < 0.05) by supplementation of G. candidum post bacterial challenge. The results showed that G. candidum is a potential probiotic as it could improve feed utilization, immunity and cause no harmful effects on growth of gibel carp at higher supplementation levels. The productive results can be achieved by using the lower dose as 10(6)-10(8) CFU/kg diet.

KeywordGibel carp Geotrichum candidum Growth Immunity Pro-inflammatory cytokines Gut microbiota
DOI10.1016/j.fsi.2020.02.001
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000523601500015
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; FEED-UTILIZATION ; PERFORMANCE ; STRAINS ; PROBIOTICS ; RESPONSES ; BACTERIA ; MORPHOLOGY ; LYSOZYME ; TLR2
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/36021
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie Shouqi
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Hubei Engn Res Ctr Aquat Anim Nutr & Feed, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Noor-Ul, Huda,Liu Haokun,Jin Junyan,et al. Dietary supplementation of Geotrichum candidum improves growth, gut microbiota, immune-related gene expression and disease resistance in gibel carp CAS III (Carassius auratus gibelio)[J]. FISH & SHELLFISH IMMUNOLOGY,2020,99(1):144-153.
APA Noor-Ul, Huda.,Liu Haokun.,Jin Junyan.,Zhu Xiaoming.,Han Dong.,...&Xie Shouqi.(2020).Dietary supplementation of Geotrichum candidum improves growth, gut microbiota, immune-related gene expression and disease resistance in gibel carp CAS III (Carassius auratus gibelio).FISH & SHELLFISH IMMUNOLOGY,99(1),144-153.
MLA Noor-Ul, Huda,et al."Dietary supplementation of Geotrichum candidum improves growth, gut microbiota, immune-related gene expression and disease resistance in gibel carp CAS III (Carassius auratus gibelio)".FISH & SHELLFISH IMMUNOLOGY 99.1(2020):144-153.
Files in This Item:
File Name/Size DocType Version Access License
1-s2.0-S105046482030(1465KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Noor-Ul, Huda]'s Articles
[Liu Haokun]'s Articles
[Jin Junyan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Noor-Ul, Huda]'s Articles
[Liu Haokun]'s Articles
[Jin Junyan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Noor-Ul, Huda]'s Articles
[Liu Haokun]'s Articles
[Jin Junyan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S1050464820300723-main.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.