IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Gut microbiota modulation and immunity response induced by Citrobacter freundii strain GC01 in grass carp (Ctenopharyngodon idellus)
Xiong, Fan1,2; Qin, Lu1,2; Hao, Yao-tong3; Zhao, Di1,2; Li, Wen-xiang1,2; Zou, Hong1,2; Li, Ming1,2; Wu, Shan-gong1,2; Wang, Gui-tang1,2
Corresponding AuthorXiong, Fan() ; Wu, Shan-gong(wusgz@ihb.ac.cn)
2020-05-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume521Pages:9
AbstractThe effect of Citrobacter freundii, an opportunistic pathogen of fish, on the gut microbiota and immune responses in fish remains unknown. We fed grass carp (Ctenopharyngodon idellus) with two different concentrations of this bacterium (experimental Low and High groups, plus Control group). No signs of acute inflammatory response were found in either of the experimental groups using the intestinal HE staining method, but the number of intestinal goblet cells was much higher in the High group. Furthermore, we found that feeding C. freundii significantly decreased the alpha diversity and altered beta diversity of gut microbiota in both groups, and significantly promoted the relative abundance of Enterobacteriales, Pasteurellales, Neisseriales and Citrobacter in the High group. Spleen transcriptome showed that the expression pattern of immune system-related genes varied significantly between the Control group and High group. Several immunity-related pathways and two infectious diseases-related pathways were significantly enriched in the High group. Many complement system genes, including c3, c4, c5, c7, c8a, c8b and c1s, were also up-regulated in the High group. Overall, our results indicate that C. freundii did not trigger acute intestinal inflammation, but it changed the diversity and population structure of gut microbiota, and triggered the host's innate mucosal immune system. The enrichment of two infectious diseases-related pathways also suggested a higher risk of infection in the High group. These results contribute to our understanding of the effects of opportunistic fish pathogens on gut microbiota and host's immune responses.
KeywordGut microbiota Citrobacter freundii Immune response Goblet cells Grass carp
DOI10.1016/j.aquaculture.2020.735015
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31872612] ; National Key R&D Program of China[2018YFD0900400] ; earmarked fund for China Agriculture Research System[CARS-45-15]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000521282400042
WOS KeywordCOMPLEMENT ; INFECTION ; STRINGTIE ; SYSTEM ; HEALTH ; HISAT
PublisherELSEVIER
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35984
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorXiong, Fan; Wu, Shan-gong
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr,State Key Lab Freshwater Ecol & Biotec, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Hebei Agr Univ, Ocean Coll, Qinhuangdao, Hebei, Peoples R China
Recommended Citation
GB/T 7714
Xiong, Fan,Qin, Lu,Hao, Yao-tong,et al. Gut microbiota modulation and immunity response induced by Citrobacter freundii strain GC01 in grass carp (Ctenopharyngodon idellus)[J]. AQUACULTURE,2020,521:9.
APA Xiong, Fan.,Qin, Lu.,Hao, Yao-tong.,Zhao, Di.,Li, Wen-xiang.,...&Wang, Gui-tang.(2020).Gut microbiota modulation and immunity response induced by Citrobacter freundii strain GC01 in grass carp (Ctenopharyngodon idellus).AQUACULTURE,521,9.
MLA Xiong, Fan,et al."Gut microbiota modulation and immunity response induced by Citrobacter freundii strain GC01 in grass carp (Ctenopharyngodon idellus)".AQUACULTURE 521(2020):9.
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