IHB OpenIR  > 水生生物多样性与资源保护研究中心  > 期刊论文
Susceptibility and immune responses after challenge with Flavobacterium columnare and Pseudomonas fluorescens in conventional and specific pathogen-free rare minnow (Gobiocypris rarus)
Su, Liangxia1,2; Xu, Chunsen1,2; Cai, Lei1,2,3; Qiu, Ning1,2; Hou, Miaomiao1; Wang, Jianwei1
Corresponding AuthorWang, Jianwei(wangjw@ihb.ac.cn)
2020-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume98Pages:875-886
AbstractThe susceptibility of fish from different culture environments to bacterial infection is not well known. The susceptibility and pathological changes of conventional (CV) and specific pathogen-free (SPF) rare minnow (Gobiocypris rarus) infected with two gram-negative bacteria, Flavobacterium columnare and Pseudomonas fluorescens are investigated. Rare minnows were intraperitoneally challenged with two bacterial species to first determine semi-lethal doses (LD50), and then with the LD50 dose, determine innate immune response. Infected rare minnows developed characteristic red bellies and then died. LD50 doses of F. columnare and P. fluorescens were 4.586 x 10(8) cfu/mL and 2.319 x 10(10) cfu/mL for CV rare minnow, and 2.575 x 10(8) cfu/mL and 1.935 x 10(10) cfu/mL, respectively, for SPF rare minnow. The results of RT-PCR showed that the highest levels of toll-like receptor 3 (TLR3), interleukin-6 (IL-6), interferon-2 (IFN-2) and rare minnow Z-DNA binding protein kinase (GrPKZ) mRNA were noticed at 6-48 h post-infection (hpi). In addition, TLR3, IL-6 and IFN-2 in F. colwnnare challenged rare minnow were more highly expressed than those in P. fluorescens challenged rare minnow, whereas as opposed in the expression of GrPKZ mRNA. Stimulation of innate immune responses is closely related to bacterial virulence. SPF rare minnow might be more susceptible to these bacteria than CV rare minnow, possibly due to their clean environment and lack of resistance. We speculate that clean environment renders rare minnow more susceptible to bacterial infections.
KeywordRare minnow Flavobacterium columnare Pseudomonas fluorescens Susceptibility Immune response
DOI10.1016/j.fsi.2019.11.047
Funding OrganizationTechnology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China
Indexed BySCI ; SCI
Language英语
Funding ProjectTechnology R & D Program of Hubei Province, China[2015BCE098]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000516880900092
WOS KeywordEXPRESSION ANALYSIS ; MOLECULAR-CLONING ; CHANNEL CATFISH ; TLR3 GENE ; CARP ; DISEASE ; INFECTION ; MODEL ; FISH
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationTechnology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35956
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorWang, Jianwei
Affiliation1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Guangdong Lab Anim Monitoring Inst, Guangzhou, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Su, Liangxia,Xu, Chunsen,Cai, Lei,et al. Susceptibility and immune responses after challenge with Flavobacterium columnare and Pseudomonas fluorescens in conventional and specific pathogen-free rare minnow (Gobiocypris rarus)[J]. FISH & SHELLFISH IMMUNOLOGY,2020,98:875-886.
APA Su, Liangxia,Xu, Chunsen,Cai, Lei,Qiu, Ning,Hou, Miaomiao,&Wang, Jianwei.(2020).Susceptibility and immune responses after challenge with Flavobacterium columnare and Pseudomonas fluorescens in conventional and specific pathogen-free rare minnow (Gobiocypris rarus).FISH & SHELLFISH IMMUNOLOGY,98,875-886.
MLA Su, Liangxia,et al."Susceptibility and immune responses after challenge with Flavobacterium columnare and Pseudomonas fluorescens in conventional and specific pathogen-free rare minnow (Gobiocypris rarus)".FISH & SHELLFISH IMMUNOLOGY 98(2020):875-886.
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